舒筋健腰丸对坐骨神经痛大鼠坐骨神经组织及血清细胞因子的影响

摘要
图/表
参考文献(20)
相关文章 (15)

全文: PDF (1268 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要目的研究舒筋健腰丸对坐骨神经痛大鼠的坐骨神经组织病理改变以及血清中细胞因子的影响。方法选取112只SD大鼠，随机分为假手术组，模型组，壮骨关节丸组，芬必得酚咖片组，舒筋健腰丸高（4.38 g/kg）、中（2.19 g/kg）、低（1.10 g/kg）剂量组，每组16只。通过钝性分离，暴露大鼠右下肢坐骨神经并对其进行4道结扎，制备坐骨神经痛模型。假手术组只暴露坐骨神经但不结扎坐骨神经。造模成功后，连续灌胃给药28 d。末次给药后，检测各组大鼠的缩爪潜伏期，血清中TNF-α、IL-1β、IL-6、PGE2的含量及坐骨神经组织的病理学改变。结果与假手术组相比，模型组大鼠缩爪潜伏期明显缩短（P < 0.05），坐骨神经发生水肿和空泡变性，血清中IL-1β、IL-6含量显著升高（P < 0.05）。与模型组相比，舒筋健腰丸组缩爪潜伏期延长（P < 0.05），坐骨神经病的变程度减轻，血清中炎性因子IL-1β、IL-6含量下降（P < 0.05）。结论舒筋健腰丸能明显减轻坐骨神经组织学改变，抑制炎性因子表达，对损伤性神经痛具有治疗作用。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	石洪超1 欧慧瑜2 何风雷1 陈迪新2 彭富全1 李瑞鹏2 莫国强1

关键词 ：舒筋健腰丸, 坐骨神经痛, 细胞因子, 组织病理

Abstract：Objective To study the effect of Shujin Jianyao Pills on the pathological changes of ischiadic nerve and cytokine in the serum of CCI (chronic constriction injury) rats. Methods 112 SD rats were randomly divided into sham operation group, model group, Zhuanggu Guanjie Pill group, Paracetamol and Caffeine Tablets group, Shujin Jianyao Pill low dose group (1.10 g/kg), middle dose group (2.19 g/kg) and high dose group (4.38 g/kg), with 16 rats in each group. The right common sciatic nerve was exposed by blunt dissection, and four ligatures were tied loosely around the sciatic nerve. Sham operation referred to the procedure of exposing the nerve as above but without any nerve ligation. All groups were administrated with corresponding drugs by intragastric administration once a day for 28 days. After the last administration, the hindpaw withdrawal latency (HWL) of the rats, the contents of inflammatory cytokines including TNF-α, IL-1β, IL-6, PGE2 in the serum and the pathological changes of ischiadic nerve were detected. Results Compared to the sham operation group, in the model group, the HWL was shortened, ischiadic nerve was become swollen and vacuolar, the serum levels of IL-1β and IL-6 were increased (P < 0.05). Compared to the model group, in the Shujin Jianyao Pill groups, the HWL were extended, the pathological changes were ameliorated induced by CCI, and the IL-1β and IL-6 levels were decreased (P < 0.05). Conclusion Shujin Jianyao Pill can alleviate the histologic changes of sciatic nerve, inhibit the expression of inflammatory cytokines, which has therapeutic effect on traumatic neuralgia.

Key words： Shujin Jianyao Pill Sciatica Cytokine Histopathology

基金资助:广东省科技计划项目（2013A032500020）。

通讯作者: 欧慧瑜（1976.8-），女，硕士，高级工程师；研究方向：药理毒理。

引用本文:

石洪超1 欧慧瑜2 何风雷1 陈迪新2 彭富全1 李瑞鹏2 莫国强1. 舒筋健腰丸对坐骨神经痛大鼠坐骨神经组织及血清细胞因子的影响[J]. 中国医药导报, 2017, 14(22): 11-15.
SHI Hongchao1 OU Huiyu2 HE Fenglei1 CHEN Dixin2 PENG Fuquan1 LI Ruipeng2 MO Guoqiang1. Effect of Shujin Jianyao Pills on ischiadic nerve and cytokine in serum of CCI rats. 中国医药导报, 2017, 14(22): 11-15.

链接本文:

http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I22/11

[1] Jay GW，Barkin RL. Neuropathic pain：etiology，pathophysiology，mechanisms，and evaluations [J]. Dis Mon，2014， 60（1）：6-47.
[2] 倪雪珺，杜冬萍.神经病理性疼痛与抑郁和焦虑精神症状的相关性[J].上海交通大学学报：医学版，2013，33（1）：67-70.
[3] Savastano LE，Laurito SR，Fitt MR，et al. Sciatic nerve injury：a simple and subtle model for investigating many aspects of nervous system damage and recovery [J]. J Neurosci Methods，2014，227：166-180.
[4] 彭晴，刘涛，余昌胤，等.淫羊藿次苷Ⅱ对大鼠坐骨神经损伤后功能恢复和神经再生的影响[J].中国医药导报，2016，13（5）：12-16.
[5] 冯慧琼，董峰.人参皂苷Rg1促进坐骨神经损伤后修复的研究[J].中国现代医生，2016，54（14）：27-30.
[6] 冯峰，邹庆玲.中药治疗糖尿病周围神经病变临床疗效观察[J].中华中医药学刊，2014，32（8）： 2008-2013.
[7] 唐萌芽，翁祝承，邵利芳.中药治疗膝骨关节炎临床疗效和安全性的系统评价[J].中医正骨，2014，26（1）：43-48.
[8] 严红梅，张振海，孙娥，等.中药治疗骨质疏松症的研究进展[J].中草药，2014，45（8）：1174-1178.
[9] 谢琳，吴启富，王笑丹，等. 舒筋健腰丸对骨关节炎大鼠关节软骨和血清中细胞因子的影响[J].2014，24（12）：862-865.
[10] 谢林，刘彦博，吴启富，等.舒筋健腰丸镇痛抗炎作用实验研究[C]//全国第十一届中西医结合风湿病学术会议，2013.
[11] Bennett GJ. What is spontaneous pain and who has it？[J]. J Pain，2012，13（10）：921-929.
[12] van Hecke O，Austin SK，Khan RA，et al. Neuropathic pain in the general population：a systematic review of epidemiological studies [J]. Pain，2014，155（4）：654-662.
[13] Hall GC，Morant SV，Carroll D，et al. An observational descriptive study of the epidemiology and treatment of neuropathic pain in a UK general population [J]. BMC Fam Pract，2013，14（1）：28.
[14] Challa SR. Surgical animal models of neuropathic pain：pros and cons [J]. Int J Neurosci，2015，125（3）：170-174.
[15] Li Q，Tian Y，Wang ZF，et al. Involvement of the spinal NALP1 inflammasome in neuropathic pain and aspirin-triggered-15-epi-lipoxin A4 induced analgesia [J]. Neuroscience，2013，254：230-240.
[16] Sacerdote P，Franchi S，Moretti S，et al. Cytokine modulation is necessary for efficacious treatment of experimental neuropathic pain [J]. J Neuroimmune Pharmacol，2013，8（1）：202-211.
[17] Gong X，Chen Y，Fu B，et al. Infant nerve injury induces delayed microglial polarization to the M1 phenotype，and exercise reduces delayed neuropathic pain by modulating microglial activity [J]. Neuroscience，2017，349：76-86.
[18] Li K，Tan YH，Light AR，et al. Different peripheral tissue injury induces differential phenotypic changes of spinal activated microglia [J]. Clin Dev Immunol，2013，2013（10）：901420.
[19] Choi HS，Roh DH，Yoon SY，et al. Microglial interleukin-1β in the ipsilateral dorsal horn inhibits the development of mirror-image contralateral mechanical allodynia through astrocyte activation in a rat model of inflammatory pain [J]. Pain，2015，156（6）：1046-1059.
[20] Murakami T，Kanchiku T，Suzuki H，et al. Anti-interleukin-6 receptor antibody reduces neuropathic pain following spinal cord injury in mice [J]. Exp Ther Med，2013，6（5）：1194-1198.