谷胱甘肽肌肤凝胶剂对大鼠皮肤浅Ⅱ度烫伤创面愈合的实验研究

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摘要目的观察谷胱甘肽肌肤凝胶剂对大鼠皮肤浅Ⅱ度烫伤创面愈合的作用。方法选取60只雄性SD大鼠，分为正常组、模型组、生长因子组、康复新组、谷胱甘肽肌肤凝胶剂低剂量组和高剂量组，每组各10只。采用75℃热水浸烫脱毛区（3 cm×3 cm）25 s，成功建立大鼠浅Ⅱ度烫伤模型。应用谷胱甘肽肌肤凝胶剂涂抹烫伤创面，并以生长因子组和康复新组为对照，给药量为1 mL/只，每天涂药2次，连续给药16 d，涂药后创面不加覆盖，逐日观察创面感染愈合情况及动物全身情况（体征表现和创面结痂、愈合情况），连续观察20 d。结果谷胱甘肽肌肤凝胶剂高剂量组对浅Ⅱ度烫伤模型大鼠可缩短创面脱痂时间和愈合时间，缩小愈合面积，与模型组比较差异有统计学意义（P < 0.05或P < 0.01）。病理组织学检查结果表明：模型组可见创面结痂，痂皮内为较多的炎性渗出物，痂皮下肉芽组织已经再生修复，创缘周围开始有少量上皮再生，并逐渐开始覆盖创面；与模型组相比，各组均有促进创面愈合的作用，炎性渗出物减少，肉芽组织更加成熟，上皮再生较为明显，谷胱甘肽肌肤凝胶剂高剂量作用与重组人碱性成纤维细胞生长因子相当。结论谷胱甘肽肌肤凝胶剂对浅Ⅱ度烫伤具有较好地促进创面愈合的作用。

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	王清1 陶继善2 朱萱萱1 张迪1 杨晨1 符蕊1

关键词 ：谷胱甘肽肌肤凝胶剂, 浅Ⅱ度烫伤模型, 病理组织学, 创面结痂, 愈合情况

Abstract：Objective To observe the effects of glutathione skin gel on wound healing of superficial second-degree burn in rats. Methods 60 SPF male SD rats were divided into normal group, model group, growth factor group, Kangfuxin group, low-dose and high-dose glutathione skin gel groups, each group had 10 rats. The superficial second-degree burn model was successfully established by scalding the hair-removed area (3 cm × 3 cm) of rats with 75℃ water bath for 25 s. The rats were subjected to application of glutathione skin gel on wound without coverage (1 mL, bid) for 16 days, and recombinant human basic fibroblast growth factor and Kangfuxin were used as control. The wound healing process and whole body conditions of animals (physical signs and scabs formation) were observed daily for 20 days. Results Compared with the model group, treatment of high-dose glutathione skin gel shortened the time of wound healing and getting rid of scabs, reduced healing area in superficial second-degree burn model of rats (P < 0.05 and P < 0.01). Histopathological examination results showed that the wound scab was visible in model group, but with excessive inflammatory exudate. Granulation tissue had been repaired and regenerated under the scabs, and small quantities of epithelia had been regenerated around the wound edge, covering the wound gradually. Compared with the model group, all other groups promoted wound healing and decreased inflammatory exudates. And regeneration of the granulation tissue and epithelia were more obvious and significant in these groups. High-dose glutathione skin gel group and growth factor group had comparable effects on the wound healing. Conclusion Glutathione skin gel significantly promotes wound healing of superficial second-degree burn in rats.

Key words： Glutathione skin gel Superficial second-degree burn model Pathological histology Wound scab Wound healing

基金资助:江苏省高校优势学科建设工程资助项目（96）。

通讯作者: 朱萱萱（1952-），女，教授，博士生导师；研究方向：中药药理学。

作者简介: 王清（1969-），女，副主任药师，主要从事中药研究工作。

引用本文:

王清1 陶继善2 朱萱萱1 张迪1 杨晨1 符蕊1 . 谷胱甘肽肌肤凝胶剂对大鼠皮肤浅Ⅱ度烫伤创面愈合的实验研究[J]. 中国医药导报, 2017, 14(31): 9-14.
WANG Qing1 TAO Jishan2 ZHU Xuanxuan1 ZHANG Di1 YANG Chen1 FU Rui1 . Experiment study of healing effects of glutathione skin gel on superficial second-degree burn wounds in rats. 中国医药导报, 2017, 14(31): 9-14.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2017/V14/I31/9

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