低密度脂蛋白胆固醇匀相法与公式法间移动相关系数的研究

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摘要目的观察血脂含量对血清低密度脂蛋白胆固醇（LDL-C）匀相法与公式法相关性的影响，比较LDL-C匀相法与五种公式法结果间的相关性，探讨公式法的临床应用价值。方法募集2014年5月～2014年7月阜外医院就诊的患者17 488例，采用贝克曼AU5421全自动生化分析仪测定载脂蛋白（ApoB）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、LDL-C和三酰甘油（TG）。利用移动相关系数（Mr）观察血脂含量对LDL-C匀相法与公式法相关性的影响，同时观察匀相法与公式法的相关性和差异性。结果 LDL-C匀相法和5种公式法的结果存在强相关（r > 0.95，P < 0.05），中位数偏差在-3.065~-8.145%，差异有统计学意义（P < 0.05），Mr与ApoB、TC和HDL-C血清含量呈同向变化，在LDL-C中先下降后升高，在TG中先恒定后下降。Delong法、胡柏成法和吴秀茹法与LDL-C匀相法相关性最强（r = 0.97，P < 0.05）。同时还发现，LDL-C与ApoB和TC呈强相关，（r = 0.92，P < 0.05）。结论公式法与匀相法测定值呈显著相关性，差异有统计学意义（P < 0.05）。如果有条件，建议实验室均采用匀相法进行检测。

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	陈宣男王志富蔺亚晖

关键词 ：低密度脂蛋白胆固醇, 移动相关系数, 匀相法, Friedewald公式

Abstract：Objective To observe the effect of lipid levels on the correlation between homogeneous and formula assays for measuring low-density lipoprotein cholesterol (LDL-C), and compare the relationship and difference between homogeneous and five formula assays, in order to explore the clinical application of formulas. Methods From May 2014 to July 2014, total 17 488 patients from Fuwai Hospital were recruited. All of apolipoprotein B (ApoB), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), LDL-C and total triglyceride (TG) were measured by an automatic biochemical analyzer (AU5421, Beckman). The moving correlation coefficients (Moving r, Mr) were computed to evaluate lipid effects on the correlation between homogeneous assay and formulas. Meanwhile, observe the correlation and the difference between different methods. Results There were strong correlations between five formulas and homogeneous method (r > 0.95, P < 0.05). Biases of median ranging were from -3.065% to -8.145%, the differences were statistically significant (P < 0.05). Mrs changed with levels of ApoB, TC and HDL-C, which decreased firstly, then increased with LDL-C level, and hold constant, then decreased with TG level. The correlations between Delong, Hu Bocheng and Wu Xiuru methods were the strongest (r = 0.97, P < 0.05). It was also found that LDL-C was correlated with ApoB and TC levels (r = 0.92，P < 0.05). Conclusion There are significant correlations as well as the differences were statistically significant (P < 0.05) between the homogeneous assay and the formulas. It is recommended that the laboratory would employ the homogeneous assay.

Key words： Low density lipoprotein cholesterol Moving correlation coefficient Homogeneous assay Friedewald formula

引用本文:

陈宣男王志富蔺亚晖. 低密度脂蛋白胆固醇匀相法与公式法间移动相关系数的研究[J]. 中国医药导报, 2018, 15(18): 163-166.
CHEN Xuannan WANG Zhifu LIN Yahui . Study of moving r between homogeneous and formula assays to test LDL-C. 中国医药导报, 2018, 15(18): 163-166.

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http://www.yiyaodaobao.com.cn/CN/ 或 http://www.yiyaodaobao.com.cn/CN/Y2018/V15/I18/163

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