Abstract:[Abstract] Objective To establish a determination method for polysaccharide in Jianpizhixie Capsules Methods Anhydrous glucose was used as reference substance. Total sugar content was determined by phenol-sulfate acid method; 1.5mol/L hydrolyzed polysaccharide sulfate solution was used, the method of alkali neutralization and ethanol precipitation was used to get simple sugars. The HPLC-ELSD method was used to determine its content. Chromatographic condition: chromatographic column was Xbridge Amide column (4.6 mm×150 mm, 3.5 μm); Mobile phase was acetonitrile-0.1%triethylamine (75∶25), column temperature was 30℃, flow rate was 1.0 mL/min, temperature of ELSD was 95℃, gas flow rate was 3.0 L/min. Results Phenol-sulfate acid method of anhydrous glucose was linear in the ranges of 6.8-68.0 μg/mL (r2 = 0.9995), the average recovery rate was 96.1%. HPLC-ELSD of anhydrous glucose was linear in the ranges of 0.0507-0.8110 mg/mL (r2 = 0.9998), the average recovery was 97.1%, RSD = 1.4%. The average contents of two kinds methods for determination of polysaccharide were 22.3%, 15.2%. Conclusion The method is stable and feasible, has good repeatability and can be used as the polysaccharide content determination of Jianpizhixie Capsules.