Expression and clinical significance of lncRNA MNX1-AS1 and miR-218-5p in non-small cell lung cancer tissues
WANG Qiyuan1 LI Chengyin1 ZHU Jing1 ZOU Yinshui1 ZHAO Yaying1 GUO Guanghui2
1.Department of Oncology，Hubei Provincial Hospital of Traditional Chinese medicine, Hubei Province, Wuhan 430061, China;
2.Department of Respiratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province, Wuhan 430061, China
Abstract：Objective To study the expression and clinical significance of long non-coding RNA (LncRNA) MNX1-AS1 and microRNA (miR)-218-5p in non-small cell lung cancer (NSCLC) tissues. Methods A total of 120 NSCLC patients diagnosed and treated in Hubei Provincial Hospital of Traditional Chinese Medicine from January 2015 to January 2017 were selected. The expression of LncRNA MNX1-AS1 and miR-218-5p were detected by fluorescence quantitative PCR from NSCLC tissues and paracancer tissues. The correlation between LncRNA MNX1-AS1 and miR-218-5p in NSCLC tissues and its clinicopathologic features were analyzed. The mean of LncRNA MNX1-AS1 and miR-218-5p relative expression levels (2.321、1.213) were divided into high and low expression groups to analyze their effects on survival and prognosis of patients with NSCLC. Results The expression of LncRNA MNX1-AS1 and miR-218-5p in NSCLC tissues was higher than that in paracancer tissues (P＜0.05). LncRNA MNX1-AS1 was negatively correlated with miR-218-5p (r=-0.561, P＜0.01). The expression of LncRNA MNX1-AS1 and miR-218-5p in patients with different lymph node metastases and tumor stages were statistically significant (P＜0.05). The survival curve of LncRNA MNX1-AS1 high-expression group was lower than that of LncRNA MNX1-AS1 low-expression group, and the survival curve of miR-218-5p low-expression group was lower than that of miR-218-5p high-expression group (P＜0.05). Conclusion The expression of LncRNA MNX1-AS1 increased and miR-218-5p decreased in NSCLC tissues, both of which are associated with poor prognosis of patients and may serve as prognostic evaluation indicators.
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