Research progress on exosome targeted delivery of nucleic acid molecules
JIA Yue1 LI Yan2 WANG Yingji1
1.Inorganic Chemistry Teaching and Research Office, School of Pharmacy, Harbin Medical University, Heilongjiang Province, Harbin 150081, China; 2.Research Center of Traditional Chinese Medicine Translational Medicine, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China
Abstract：Exosomes, as small nanoscale extracellular vesicles are an important mode of cellular information delivery in living organisms. Exosomes are characterized by high delivery efficiency, low immunogenicity, the ability to cross multiple biological barriers, and targeting properties. This has made exosomes an important member of drug delivery carriers to overcome the disadvantages of poor stability, insufficient bioavailability, low solubility, and high toxicity of some drugs. This article reviews the use of exosomes in vesicle form as novel drug delivery molecules, including microRNA, small interfering RNA and deoxyribo nucleic acid, which have received much attention in recent years. This article aims to provide a theoretical basis and design ideas for the development of efficient and safe delivery of exosome targeted nucleic acid like molecules and to facilitate their clinical translation.
[1] Johnstone RM. Exosomes biological significance：a concise review [J]. Blood Cells Mol Dis，2006，36（2）：315-321.
[2] Salari N，Rasoulpoor S，Valipour E，et al. Liposomes，new carriers for delivery of genes and anticancer drugs：a systematic review [J]. Anticancer Drugs，2022，33（1）：e9-e20.
[3] Grigoletto A，Tedeschini T，Canato E，et al. The evolution of polymer conjugation and drug targeting for the delivery of proteins and bioactive molecules [J]. Wiley Interdiscip Rev Nanomed Nanobiotechnol，2021，13（3）：e1689.
[4] Mitchell MJ，Billingsley MM，Haley RM，et al. Engineering precision nanoparticles for drug delivery [J]. Nat Rev Drug Discov，2021，20（2）：101-124.
[5] Rufino-Ramos D，Albuquerque PR，Carmona V，et al. Extracellular vesicles：novel promising delivery systems for therapy of brain diseases [J]. J Control Release，2017，262：247-258.
[6] Mcandrews KM，Kalluri R. Mechanisms associated with biogenesis of exosomes in cancer [J]. Mol Cancer，2019，18（1）：52.
[7] Zhou Y，Tian T，Zhu Y，et al. Exosomes transfer among different species cells and mediating miRNAs delivery [J]. J Cell Biochem，2017，118（12）：4267-4274.
[8] Farooqi AA，Desai NN，Qureshi MZ，et al. Exosome biogenesis，bioactivities and functions as new delivery systems of natural compounds [J]. Biotechnol Adv，2018，36（1）：328- 334.
[9] Théry C，Zitvogel L，Amigorena S. Exosomes：composition，biogenesis and function [J]. Nat Rev Immunol，2002，2（8）：569-579.
[10] Holliday LS，Faria L，Rody WJ. Actin and actin-associated proteins in extracellular vesicles shed by osteoclasts [J]. Int J Mol Sci，2019，21（1）：158.
[11] Li X，Liang Z，Du J，et al. Herbal decoctosome is a novel form of medicine [J]. Sci China Life Sci，2019，62（3）：333-348.
[12] Luan X，Sansanaphongpricha K，Myers I，et al. Engineering exosomes as refined biological nanoplatforms for drug delivery [J]. Acta Pharmacol Sin，2017，38（6）：754-763.
[13] Zhang M，Viennois E，Prasad M，et al. Edible ginger-derived nanoparticles：a novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer [J]. Biomaterials，2016，101：321- 340.
[14] Alenquer M，Amorim MJ. Exosome biogenesis，regulation，and function in viral infection [J]. Viruses，2015，7（9）：5066-5083.
[15] Kalluri R，Lebleu VS. The biology，function，and biomedical applications of exosomes [J]. Science，2020，367（6478）：eaau6977.
[16] Marazioti A，Papadia K，Kannavou M，et al. Cellular vesicles：new insights in engineering method，interaction with cells and potential for brain targeting [J]. J Pharmacol Exp Ther，2019，370（3）：772-785.
[17] Yousefpour P，Chilkoti A. Co-opting biology to deliver drugs [J]. Biotechnol Bioeng，2014，111（9）：1699-1716.
[18] Goh WJ，Zou S，Ong WY，et al. Bioinspired cell-derived nanovesicles versus exosomes as drug delivery systems：a cost-effective alternative [J]. Sci Rep，2017，7（1）：14322.
[19] Hill M，Tran N. miRNA interplay：mechanisms and consequences in cancer [J]. Dis Model Mech，2021，14（4）：dmm047662.
[20] Jeong K，Yu YJ，You JY，et al. Exosome-mediated microRNA-497 delivery for anti-cancer therapy in a microfluidic 3D lung cancer model [J]. Lab Chip，2020，20（3）：548- 557.
[21] Lou G，Song X，Yang F，et al. Exosomes derived from miR- 122-modified adipose tissue-derived MSCs increase chemosensitivity of hepatocellular carcinoma [J]. J Hematol Oncol，2015，8：122.
[22] Lou G，Chen L，Xia C，et al. MiR-199a-modified exosomes from adipose tissue-derived mesenchymal stem cells improve hepatocellular carcinoma chemosensitivity through mTOR pathway [J]. J Exp Clin Cancer Res，2020，39（1）：4.
[23] Wang F，Hongwei T，Li L，et al. Exosome miR-335 as a novel therapeutic strategy in hepatocellular carcinoma [J]. Hepatology，2018，67（3）：940-954.
[24] Lopatina T，Grange C，Fonsato V，et al. Extracellular vesicles from human liver stem cells inhibit tumor angiogenesis [J]. Int J Cancer，2019，144（2）：322-333.
[25] Chan JA，Krichevsky AM，Kosik KS. MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells [J]. Cancer Res，2005，65（14）：6029-6033.
[26] Chuang HY，Su YK，Liu HW，et al. Preclinical evidence of STAT3 inhibitor pacritinib overcoming temozolomide resistance via downregulating miR-21-enriched exosomes from M2 glioblastoma-associated macrophages [J]. J Clin Med，2019，8（7）：959.
[27] Wong S，Zhang X，Zhuang J，et al. MicroRNA-21 inhibition enhances in vitro chemosensitivity of temozolomide-resistant glioblastoma cells [J]. Anticancer Res，2012，32（7）：2835-2841.
[28] Li B，Cao Y，Sun M，et al. Expression，regulation，and function of exosome-derived miRNAs in cancer progression and therapy [J]. FASEB J，2021，35（10）：e21916.
[29] Wang JJ，Wang ZY，Rui C，et al. Macrophage-secreted exosomes delivering miRNA-21 inhibitor can regulate BGC- 823 cell proliferation [J]. Asian Pac J Cancer Prev，2015， 16（10）：4203-4209.
[30] Munoz JL，Bliss SA，Greco SJ，et al. Delivery of functional anti-miR-9 by mesenchymal stem cell-derived exosomes to glioblastoma multiforme cells conferred chemosensitivity [J]. Mol Ther Nucleic Acids，2013，2（10）：e126.
[31] Sun L，Zhu M，Feng W，et al. Exosomal miRNA Let-7 from menstrual blood-derived endometrial stem cells alleviates pulmonary fibrosis through regulating mitochondrial DNA damage [J]. Oxid Med Cell Longev，2019，2019：4506303.
[32] Kadota T，Fujita Y，Araya J，et al. Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk [J]. J Extracell Vesicles，2021，10（10）：e12124.
[33] Shtam TA，Kovalev RA，Varfolomeeva EY，et al. Exosomes are natural carriers of exogenous siRNA to human cells in vitro [J]. Cell Commun Signal，2013，11：88.
[34] Wang C，Chen L，Huang Y，et al. Exosome delivered TRPP2 siRNA inhibits the epithelial mesenchymal transition of FaDu cells [J]. Oncol Lett，2019，17（2）：1953-1961.
[35] Nievergall E，Ramshaw HS，Yong AS，et al. Monoclonal antibody targeting of IL-3 receptor a with CSL362 effectively depletes CML progenitor and stem cells [J]. Blood，2014， 123（8）：1218-1228.
[36] Bellavia D，Raimondo S，Calabrese G，et al. Interleukin 3-receptor targeted exosomes inhibit in vitro and in vivo chronic myelogenous leukemia cell growth [J]. Theranostics，2017，7（5）：1333-1345.
[37] Yang T，Fogarty B，Laforge B，et al. Delivery of small interfering RNA to inhibit vascular endothelial growth factor in zebrafish using natural brain endothelia cell-secreted exosome nanovesicles for the treatment of brain cancer [J]. AAPS J，2017，19（2）：475-486.
[38] Didiot MC，Hall LM，Coles AH，et al. Exosome-mediated delivery of hydrophobically modified siRNA for huntingtin mRNA silencing [J]. Mol Ther，2016，24（10）：1836-1847.
[39] Thakur KB，Zhang H，Becker A，et al. Double-stranded DNA in exosomes：a novel biomarker in cancer detection [J]. Cell Res，2014，24：766-769.
[40] Yang S，Che S，Kurywchak P，et al. Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer [J]. Cancer Biol Ther，2017，18（3）：158-165.
[41] Li J，Huang S，Zhou Z，et al. Exosomes derived from rAAV/ AFP-transfected dendritic cells elicit specific T cell-mediated immune responses against hepatocellular carcinoma [J]. Cancer Manag Res，2018，10：4945-4957.
[42] Usman WM，Pham TC，Kwok YY，et al. Efficient RNA drug delivery using red blood cell extracellular vesicles [J]. Nat Commun，2018，9（1）：2359.
京公网安备 11010502046598号