Effect of acupotomy on the cartilage matrix degradation in knee osteoarthristis rabbit model of knee osteoarthristis based on tissue inhibitor of metalloproteinase and matrix metalloproteinases balance
ZHANG Liangzhi1 LI Yangyang2 LIU Hong1 ZHANG Zesheng3 CHEN Bin1 XIE Ziyi2 LIU Jing1 XIU Zhongbiao1
1.The Second Department of Orthopedics-traumatology, the People’s Hospital Affiliated of Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350004, China;
2.College of Traditional Chinese Medicine, Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350122, China;
3.Department of Orthopedics-traumatology, Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350003, China
Abstract：Objective To investigate the effect of acupotomy on cartilage matrix degradation in rabbit model of knee osteoarthristis(KOA) based on the balance between tissue inhibitor of metalloproteinase (TIMP) and matrix metalloproteinases (MMP). Methods Twenty-four healthy male New Zealand rabbits, aged six months and weighing (2.0±0.5) kg were divided into blank group, model group, and acupotomy group by random number table method, with eight rabbits in each group. The KOA rabbit model group was prepared by the modified Videman method, the left hind limb was immobilized with extended plaster for 6 weeks. Acupotomy group was treated four times with acupotomy once a week to loosen tendon nodules. The blank group and model group made the same grab, but does not take any intervention. One week after the intervention, HE staining morphology and saffron solid green staining were used to observe the pathological changes of rabbit cartilage in each group. The expression levels of Collagen Ⅱ, aquaporins（AQP）3, and MMP13 in chondrocytes were detected by RT-PCR, while the expression levels of Collagen Ⅱ, AQP3, TIMP1, and MMP13 were detected by Western blot. Results Compared with blank group, the appearance of cartilage in model group was uneven, the chondrocytes gathered irregularly, some necrotic cells disintegrated, the tide line disorganized, and some broke. The number of chondrocytes decreased significantly, the saffron in cartilage matrix decreased significantly, some cartilage defects, and the tide line was blurred and unclear. Compared with the model group, the appearance of cartilage in acupotomy group was smoother, the structure level was clearer, the cells tended to be neatly arranged, and the tide line was more complete than that in the model group. Not only the amount of cartilage increased, the saffranine coloring in the cartilage matrix was normal, but also the appearance of the cartilage was relatively smooth and smooth, and the tide line was relatively complete. Compared with blank group, the expression of Collagen Ⅱ mRNA in cartilage tissues of model group was down-regulated, while the expressions of AQP3, TIMP1 and MMP13 mRNA were up-regulated (P＜0.05). Compared with model group, the expression of Collagen Ⅱ mRNA in cartilage tissues in acupotomy group was up-regulated, while the expressions of AQP3, TIMP1 and MMP13 mRNA were down-regulated (P＜0.05). Compared with blank group, the expression of Collagen Ⅱ protein in cartilage tissue of model group was down-regulated, while the expressions of AQP3, TIMP1 and MMP13 protein were up-regulated (P＜0.05). Compared with model group, the expressions of Collagen Ⅱprotein in cartilage tissues of acupotomy group were up-regulated, while the expressions of AQP3 and MMP13 protein were down-regulated (P＜0.05). There was no significant difference in TIMP1 protein between acupotomy group and model group (P＞0.05). Compared with blank group, behavioral scores in model group were significantly increased (P＜0.05). Compared with the model group, the behavioral scores of acupotomy group were significantly decreased (P＜0.05). Conclusion By regulating the balance of TIMP/MMP, acupuncture treatment can slow down the degradation of cartilage extracellular matrix, thus effectively reducing the level of KOA inflammation.
张良志1 李阳阳2 刘洪1 张泽升3 陈斌1 谢梓毅2 刘晶1 修忠标1. 基于金属蛋白酶抑制因子与基质金属蛋白酶之间的平衡探讨针刀对兔膝骨关节炎模型软骨基质降解的影响[J]. 中国医药导报, 2023, 20(28): 14-19,65.
ZHANG Liangzhi1 LI Yangyang2 LIU Hong1 ZHANG Zesheng3 CHEN Bin1 XIE Ziyi2 LIU Jing1 XIU Zhongbiao1. Effect of acupotomy on the cartilage matrix degradation in knee osteoarthristis rabbit model of knee osteoarthristis based on tissue inhibitor of metalloproteinase and matrix metalloproteinases balance. 中国医药导报, 2023, 20(28): 14-19,65.
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