Abstract：Objective To identify the main chemical constituents and components absorbed into blood of Yiqi Shengjin Bag Tea by HPLC-Q-TOF-MS technology. Methods Six eight-week-old SPF male SD rats, weighing 280-300 g, were divided into administration group and control group by random number table method, with three rats in each group. In administration group, 4 ml of Yiqi Shengjin Bag Tea water extract was intragastric (the crude drug concentration was 1 g/ml), while in blank group, 4 ml of normal saline was intragastric, twice a day for continuous administration, and blood samples were collected from abdominal aorta 2 h after administration on the morning of the fourth day to prepare serum samples. A Waters Xselect HSS T3 C18 chromatographic column (50 mm×2.1 mm, 2.5 μm) was used to collect the chemical components of Yiqi Shengjin Bag Tea water extract and serum samples under positive and negative ion modes; gradient elution was performed with 0.1% formic acid-acetonitrile as mobile phase. Results Seventeen compounds were identified from Yiqi Shengjin Bag Tea water extract, mainly ginsenosides. Meanwhile, eight chemical constituents were identified from the serum sample, which were betaine, ginsenoside Rg1, ginsenoside Re, harpagoside, pseudoginsenoside F11, ginsenoside Rb1, ginsenoside Rd, and erianin. Conclusion HPLC- Q-TOF-MS technology was used to analyze the main chemical components of Yiqi Shengjin Bag Tea, which can help to reveal pharmacodynamic material basis of Shengjin Bag Tea, and provide reference for further quality control, pharmacology, and activity mechanism research.
王宏蕾 肖薇 吴剑坤. 基于HPLC-Q-TOF-MS技术分析益气生津袋泡茶的化学成分及入血成分[J]. 中国医药导报, 2023, 20(27): 30-34.
WANG Honglei XIAO Wei WU Jiankun. Analyses of chemical constituents and components absorbed into blood of Yiqi Shengjin Bag Tea by HPLC-Q-TOF-MS. 中国医药导报, 2023, 20(27): 30-34.
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