Effect of the acupuncture and moxibustion in the neurological function and treatment of injury in rats with acute intracranial hemorrhage
MA Xuemiao1 DU Ye2 CHENG Guangyu3 CHENG Weiping4
1.School of Graduate, Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
2.Department of Endocrinology, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China; 3.Department of Perception and Control, the First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Heilongjiang Province, Harbin 150040, China;
4.Department of Neurology, the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Heilongjiang Province, Harbin 150040, China
[Abstract]
Abstract：Objective To observe the improvement effect of acupuncture on the neurological function and craniocerebral injury in rats with acute intracranial hemorrhage. Methods A total of 45 healthy male SD rats of about 220 g in 6-8 weeks were selected, and they were divided into sham operation group, model group and acupuncture group by random number table method, with 15 rats in each group; each group was divided into three points at 1, 3 d and 7 d, with 5 rats in each time. The cerebral hemorrhage rat model group was established by autologous blood injection; the sham operation group only drilled holes without injecting autologous blood; the acupuncture group adopted the treatment method of piercing from Baihui to Qubin. Ludmila Belayev’s neurological function score was used to evaluate the degree of neurological deficit in rats in each group. Western blot was used to detect the expression of CTRP3 and GPX4 proteins in brain tissue. The mitochondrial morphology of nerve cells in the brain tissue of rats in each group was observed by transmission electron microscope. qRT-PCR method was used to detect the specific expression of GFAP and EGFR mRNA in rat brain tissue. Results Compared with sham operation group, neurological function deficit score in model group increased at each time point (P＜0.05). Compared with model group, neurological impairment scores of acupuncture group decreased at 1 and 3 d, the differences were statistically significant (P＜0.05), but there was no statistically significant difference in systemic impairment score at 7 d(P＞0.05). Compared with sham operation group, levels of CTRP3 and GPX4 protein in model group were decreased at each time point, and the differences were statistically significant (P＜0.05). Compared with model group, CTRP3 and GPX4 protein expression levels in acupuncture group were up-regulated at each time point, and the differences were statistically significant (P＜0.05). Compared with sham operation group, mRNA levels of GFAP and EGFR in brain tissue of rats in model group increased at each time point, and the differences were statistically significant (P＜0.05). Compared with model group, mRNA levels of GFAP and EGFR in brain tissue of rats in acupuncture group were significantly decreased at each time point, and the differences were statistically significant (P＜0.05). Conclusion Acupuncture treatment can up-regulate the expression of CTRP3 and GPX4 proteins in brain tissue, reduce the mRNA levels of GFAP and EGFR, and restore the integrity of neuronal mitochondria, thereby improving the neuromuscular injury and neurological function of rats with acute cerebral hemorrhage, and play a protective role.
马学淼1 杜晔2 程光宇3 程为平4. 针刺对急性颅内出血大鼠的神经功能及损伤的影响[J]. 中国医药导报, 2023, 20(17): 14-18.
MA Xuemiao1 DU Ye2 CHENG Guangyu3 CHENG Weiping4. Effect of the acupuncture and moxibustion in the neurological function and treatment of injury in rats with acute intracranial hemorrhage. 中国医药导报, 2023, 20(17): 14-18.
[1] 袁海潮，王秋芳，吴艳，等.中青年和老年急性脑出血患者致病原因及发病影响因素比较[J].华南预防医学，2021，47（9）：1214-1217.
[2] Van ASCH CJJ，Luitse MJA，Rinkel GE，et al. Incidence，case fatality，and functional outcome of intracerebral haemorrhage overtime，according to age，sex，and ethnic origin：a systematic review and meta-analysis [J]. Lancet Neurology，2010，9（2）： 167-176.
[3] Lee MS，Choi TY，Park JE，et al. Moxibustion for cancer care：a systematic review and meta-analysis [J]. BMC Cancer，2010，10：130.
[4] Li LX，Zhang MM，Zhang Y，et al. Acupuncture for cerebral palsy： a meta-analysis of randomized controlled trials [J]. Neural Regen Rese，2018，13（6）：1107-1117.
[5] Wang F，Lei HS，Zhao X，et al. Effect of penetrative needling of scalp-acupoints on cerebral N-acetylaspartate and choline levels in intracerebral hemorrhage rabbits [J]. Zhen Ci Yan Jiu，2011，36（4）： 242-246.
[6] Ko CN，Lee IW，Cho SY，et al. Acupuncture for Cerebral Vasospasm After Subarachnoid Hemorrhage： A Retrospective Case-Control Study [J]. J Altern Complement Med，2013， 19（5）：471-473.
[7] Chen JC. The effects of acupuncture and traditional Chinese medicines on apoptosis of brain tissue in a rat intracerebral hemorrhage model [J]. Physiol Behav，2015，151：421-425.
[8] Feng R，Zhang F. The neuroprotective effect of electro-ac- upuncture against ischemic stroke in animal model： a review [J]. Afr J Tradit Complement Altern Med，2014，11（3）：25-29.
[9] Hu X，Li B，Wang X. Scalp acupuncture therapy combined with exercise can improve the ability of stroke patients to participate in daily activities [J]. Complement Ther Clin Pract，2021，43：101343.
[10] Nam MH，Ahn KS，Choi SH. Acupuncture Stimulation Induces Neurogenesis in Adult Brain [J]. Int Rev Neurobiol，2013，111：67-90.
[11] 许文婷，薛玉满，王策，等.百会透曲鬓调控EGFR/ STAT3通路抑制脑出血大鼠神经功能损伤的机制研究[J].针刺临床杂志，2022，38（7）：70-75.
[12] Sun H，Tang Y，Li L，et al. Effects of local hypothermia on neuronal cell apoptosis after intracerebral hemorrhage in rats [J]. J Nutr Health Aging，2015，19（3）：291-298.
[13] 杜春亮，李娜，赵云洁，等.NT-proBNP、IGF-1、HIF-1α水平与脑出血患者预后的关系[J].检验医学与临床，2017，14（18）：4.
[14] 钱红，刘理静，李艳春，等.EGFR siRNA通过阻碍STAT3磷酸化抑制大鼠星形胶质细胞活化[J].中国药理学通报，2016，32（2）：216-222.
[15] 伍正彬，罗大林，蒋东坡.干扰表皮生长因子受体表达对脑出血后星形胶质细胞活化的影响及其机制[J].实用临床医药杂志，2017，21（13）：6.
[16] 肖霖，刘珍，武衡.大鼠脑出血后活化星形胶质细胞EGFR- JAK1/STAT3表达及Genistein的抑制作用[J].中风与神经疾病杂志，2015，32（10）：4.
[17] Liu B，Neufeld AH. Activation of epidermal growth factor receptor causes astrocytes to form cribriform structures [J]. Glia，2004，46（2）：153-168.
[18] Bullock R，Mendelow AD，Teasdale GM，et al. Intracranial haemorrhage induced at arterial pressure in the rat. Part 1： Description of technique，ICP changes and neuropathological findings [J]. Neurol Res，1984，6（4）：184-188.
[19] 郑希院，翟洁敏，李会琪，等.人参多糖对脑出血模型大鼠的干预效果及对海马区细胞凋亡的影响[J].疑难病杂志，2020，19（4）：5.
[20] 沈洁，沈梅红，张建斌.《实验针刺学》实验教学现状及探索[J].中国中医药现代远程教育，2013，11（14）： 58-59.
[21] Belayev L，Alonso OF，Busto R，et al. Middle Cerebral Artery Occlusion in the Rat by Intraluminal Suture： Neurological and Pathological Evaluation of an Improved Model [J]. Stroke，1996，27（9）：1616-1623.
[22] Zheng GQ，Zhao ZM，Wang Y，et al. Meta-Analysis of Scalp Acupuncture for Acute Hypertensive Intracerebral Hemorrhage [J]. J Altern Complement Med，2011，17（4）：293-299.
[23] 赵蕾，陶雪，代宇杉，等.针刺治疗中风病与脑功能重塑的研究进展[J].云南中医中药杂志，2020，41（2）：94-96.
[24] Li ZW，Zheng XN，Li P. Time-effect relationship of acupuncture on histopathology，ultrastructure，and neuroethology in the acute phase of cerebral hemorrhage [J]. Neural Regen Res，2019，14（1）：107-113.
[25] 肖霖.EGFR-JAK1/STAT3信号通路在大鼠脑出血后星形胶质细胞活化中的作用[D].衡阳：南华大学，2012.
[26] 戴晓红.基于Nrf2-ARE信号通路研究“百会”透“曲鬓”对脑出血大鼠的脑保护作用[D].哈尔滨：黑龙江中医药大学，2018.
[27] 韩佳炜，李桂平，郑健刚.针刺“人中、内关”组穴对脑出血后脑红蛋白表达动态变化及线粒体形态结构变化影响实验研究[J].辽宁中医药大学学报，2019，21（4）：170- 173.
[28] 邹伟，张国威，刘芳，等.针刺百会透曲鬓穴对脑出血大鼠脑组织形态学影响的实验研究[J].针刺临床杂志，2007（11）：41-44，62.
京公网安备 11010502046598号