Methods for the detection of circulating tumor cells #br#
SONG Yuhang1 YANG Xiaolin2 LIU Kang1 SONG Guiqin2
1.Institute of Tissue Engineering and Stem Cell Research, Nanchong Central Hospital the Second Clinical Medical College of North Sichuan Medical College, Sichuan Province, Nanchong 637000, China;
2.Biology Teaching and Research Section, School of Basic Medicine, North Sichuan Medical College, Sichuan Province, Nanchong 637000, China
Abstract：Circulating tumor cells (CTCs) carry important information about tumor genesis, development, and metastasis and are closely associated with tumor metastasis. In order to understand the function and molecular characteristics of CTCs and develop effective surveillance and treatment strategies for cancer, the development of CTCs enrichment and identification technology is a key step. In recent years, great progress has been made in the detection of CTCs in vitro, at the same time, real-time, non-invasive in vivo detection technology has gradually emerged in the market. This paper summarizes the methods of CTCs detection in vitro and in vivo, and puts forward the challenges and strategies of CTCs detection.
宋宇航1 杨小林2 刘康1 宋桂芹2. 循环肿瘤细胞的检测方法[J]. 中国医药导报, 2022, 19(6): 39-42.
SONG Yuhang1 YANG Xiaolin2 LIU Kang1 SONG Guiqin2. Methods for the detection of circulating tumor cells #br#. 中国医药导报, 2022, 19(6): 39-42.
[1] Vasilaki D，Bakopoulou A，Tsouknidas A，et al. Biophysical interactions between components of the tumor microenvironment promote metastasis [J]. Biophys Rev，2021， 13（3）：339-357.
[2] 张惠敏，吴玲玲，林冰倩，等.基于微流控技术的循环肿瘤细胞分析研究进展[J].中国科学：化学，2019，49（9）：1220-1236.
[3] Zhang H，Lin X，Huang Y，et al. Detection Methods and Clinical Applications of Circulating Tumor Cells in Breast Cancer [J]. Front Oncol，2021，11：652253.
[4] Huang Q，Wang FB，Yuan CH，et al. Gelatin Nanoparticle-Coated Silicon Beads for Density-Selective Capture and Release of Heterogeneous Circulating Tumor Cells with High Purity [J]. Theranostics，2018，8（6）：1624-1635.
[5] Hao SJ，Wan Y，Xia YQ，et al. Size-based separation methods of circulating tumor cells [J]. Adv Drug Deliv Rev，2018，125：3-20.
[6] Sun N，Li X，Wang Z，et al. High-purity capture of CTCs based on micro-beads enhanced isolation by size of epithelial tumor cells（ISET）method [J]. Biosens Bioelectron，2018，102：157-163.
[7] Barr J，Chudasama D，Rice A，et al. Lack of association between Screencell-detected circulating tumour cells and long-term survival of patients undergoing surgery for non-small cell lung cancer：A pilot clinical study [J]. Mol Clin Oncol，2020，12（3）：191-195.
[8] Choi MK，Kim GH，I H，et al. Circulating tumor cells detected using fluid-assisted separation technique in eso-phageal squamous cell carcinoma [J]. J Gastroenterol Hepatol，2019，34（3）：552-560.
[9] Alix-Panabières C，Pantel K. Challenges in circulating tumour cell research [J]. Nat Rev Cancer，2014，14（9）：623-631.
[10] 李蜀婧，王杨，张克勤.循环肿瘤细胞检测技术研究进展[J].免疫学杂志，2020，36（5）：457-460.
[11] 王鑫，黄静，刘烨磊，等.循环肿瘤细胞在胰腺癌诊治中的研究进展[J].现代实用医学，2021，33（1）：129-131.
[12] 王乐，张志强，陈建松，等.循环肿瘤细胞上皮-间质转化对肿瘤发生发展的影响及其临床应用潜力[J].福建医科大学学报，2021，55（2）：157-161.
[13] 蒲思思，周朝辉，贾能勤.循环肿瘤细胞分离富集与分析检测技术的研究进展[J].上海师范大学学报：自然科学版，2020，49（2）：219-233.
[14] Woestemeier A，Harms-Effenberger K，Karstens KF，et al. Clinical Relevance of Circulating Tumor Cells in Esop-hageal Cancer Detected by a Combined MACS Enrichment Method [J]. Cancers（Basel），2020，12（3）：718.
[15] Ahmed MG，Abate MF，Song Y，et al. Isolation，Detection，and Antigen-Based Profiling of Circulating Tumor Cells Using a Size-Dictated Immunocapture Chip [J]. Angew Chem Int Ed Engl，2017，56（36）：10681-10685.
[16] 王萍，井丰军.循环肿瘤细胞的检测与临床应用[J].检验医学与临床，2021，18（12）：1802-1805.
[17] Ramirez AB，Bhat R，Sahay D，et al. Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining，image acquisition，and single cell retrieval and analysis [J]. BMC Cancer，2019，19（1）： 220.
[18] 靳博，向征.循环肿瘤细胞在结直肠癌中的应用及研究进展[J].现代医药卫生，2021，37（14）：2416-2420.
[19] 汪裕伟，曹玉涵.尿液中哺乳动物雷帕霉素靶蛋白mRNA表达量与IgA肾病患者肾脏纤维化的相关性研究[J].皖南医学院学报，2020，39（3）：217-221.
[20] Kim TH，Wang Y，Oliver CR，et al. A temporary indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells [J]. Nat Commun，2019，10（1）：1478.
[21] Aceto N. Fluctuating numbers of circulating tumor cells in cancer patients and the meaning of zero counts [J]. Oncotarget，2019，10（28）：2658-2659.
[22] Suo Y，Gu Z，Wei X. Advances of In Vivo Flow Cytometry on Cancer Studies [J]. Cytometry A，2020，97（1）：15-23.
[23] Zhu X，Suo Y，Fu Y，et al. In vivo flow cytometry reveals a circadian rhythm of circulating tumor cells [J]. Light Sci Appl，2021，10（1）：110.
[24] 杨萍，魏丹，庞恺，等.在体光声流式细胞术在循环肿瘤细胞检测中的研究进展[J].激光与光电子学进展，2017，54（9）：7-18.
[25] 程序.非小细胞肺癌循环肿瘤细胞中EpCAM及CK19的检测及意义[D].北京：北京市结核病胸部肿瘤研究所，2018.
[26] 张文杰，张冰凯，董朔晖，等.循环肿瘤细胞在结直肠癌治疗中的研究现状[J].腹腔镜外科杂志，2018，23（8）：628-632.
[27] 孙文静，施振强，卿光焱.循环肿瘤细胞捕获材料的研究进展[J].色谱，2021，39（10）：1-4.
[28] Keller L，Pantel K. Unravelling tumour heterogeneity by single-cell profiling of circulating tumour cells [J]. Nat Rev Cancer，2019，19（10）：553-567.
[29] Genna A，Vanwynsberghe AM，Villard AV，et al. EMT-Associated Heterogeneity in Circulating Tumor Cells： Sticky Friends on the Road to Metastasis [J]. Cancers（Basel），2020，12（6）：1632
[30] Liu Q，Zhang H，Jiang X，et al. Factors involved in cancer metastasis： a better understanding to “seed and soil” hypothesis [J]. Mol Cancer，2017，16（1）：176.
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