Determination of Fluconazole concentration in neonatal plasma by ultra performance liquid chromatography-tandem mass spectrometry#br#
QIN Dongjie1* LI Ping2* WU Jing1 LIANG Jing3 WEI Yanfei3 JIANG Yongjiang3 YU Qiaoai4 HE Jing2 WANG Honghong2
1.Pharmaceutical Division, Liuzhou Quality Inspection and Testing Research Center, Guangxi Zhuang Autonomous Region, Liuzhou 545006, China; 2.Department of Pharmacy, Liuzhou Maternity and Child Healthcare Hospital Affiliated Maternity Hospital and Affiliated Children’s Hospital of Guangxi University of Science and Technology, Guangxi Zhuang Autonomous Region, Liuzhou 545001, China;
3.Department of Neonatology, Liuzhou Maternity and Child Healthcare Hospital Affiliated Maternity Hospital and Affiliated Children’s Hospital of Guangxi University of Science and Technology, Guangxi Zhuang Autonomous Region, Liuzhou 545001, China;
4.Medical Laboratory, Liuzhou Maternity and Child Healthcare Hospital Affiliated Maternity Hospital and Affiliated Children’s Hospital of Guangxi University of Science and Technology, Guangxi Zhuang Autonomous Region, Liuzhou 545001, China
Abstract：Objective To establish a method for rapid determination of Fluconazole concentration in neonatal plasma. Methods Plasma was treated with acetonitrile precipitated protein with phenacetin as internal standard. Chromatographic column: Eclipse Plus C18 RRHD (2.1 mm×100 mm, 1.8 μm); mobile phase: gradient elution of 0.1% formic acid-methanol; flow rate: 0.3 ml/min; injection volume: 1 μl. Electrochemical spray ion source positive ion mode ionization, multi-reaction monitoring mode detection, quantitative analyse the ionization pairs as mass-to-charge ratio (m/z) 307.1→220.0 (Fluconazole) and m/z 180.1→110.0 (phenacetin). Results The method had a good linearity in the range of 0.1001-60.0600 μg/ml, the lower limit of quantification was 0.01 μg/ml, the accuracy were 82.5%-101.2%, and the extraction recoveries were 85.0%-109.0%. Conclusion The method is simple, rapid, accurate, and high sensitive, suitable for determination of Fluconazole concentration in neonatal plasma.
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