Abstract：Objective To study the effect of Shenxiong Glucose Injection on the permeability of blood-cerebrospinal fluid barrier in hypoperfusion rats. Methods Sixty SPF male SD rats were divided into sham operation group, hypoperfusion group and Shenxiong group by random number table method, with 20 rats in each group. In the hypoperfusion group and Shenxiong group, the common carotid artery was ligation to make an animal model of cerebral ischemia, while in the sham operation group, only the common carotid artery was exposed without ligation. Shenxiong group was intraperitoneally injected with Shenxiong Glucose Injection 24 mg/kg, sham operation group and hypoperfusion group were intraperitoneally injected with normal saline. Serum albumin (SAlb) and cerebrospinal fluid albumin (CAlb) were determined by enzyme linked immunosorbent assay. The mRNA of ZO-1 and Occludin in choroid plexus was detected by real-time fluorescence quantitative PCR. The fluorescence intensity of ZO-1 and Occludin in choroid plexus was detected by laser confocal method. Results CAlb and serum albumin and cerebrospinal fluid quotient (QAlb) in the hypoperfusion group were higher than those in the sham operation group, and CAlb and QAlb in the Shenxiong group were lower than those in the hypoperfusion group, with statistical significance (P ＜ 0.05). There was no significant difference in SAlb between the hypoperfusion group and sham operation group (P ＞ 0.05). There was no significant difference in SAlb between the Shenxiong group and hypoperfusion group (P ＞ 0.05). The mRNA and fluorescence intensity of ZO-1 and Occludin in the hypoperfusion group were lower than those in the sham operation group, and the mRNA and fluorescence intensity of ZO-1 and Occludin in the Shenxiong group were higher than those in the hypoperfusion group, with statistical significance (P ＜ 0.05). Conclusion The permeability of blood-cerebrospinal fluid barrier increased in rats under hypoperfusion state, and Shenxiong Glucose Injection had protective effect on blood-cerebrospinal fluid barrier to some extent.
冯佩佩 张倩影 黄炎 李永杰 刘坤 李敏. 参芎葡萄糖注射液对低灌注大鼠血-脑脊液屏障通透性的影响[J]. 中国医药导报, 2021, 18(31): 37-41.
FENG Peipei ZHANG Qianying HUANG Yan LI Yongjie LIU Kun LI Min. Effect of Shenxiong Glucose Injection on permeability of blood-cerebrospinal fluid barrier in hypoperfusion rats#br#. 中国医药导报, 2021, 18(31): 37-41.
[1] Sweeney MD，Zhao Z，Montagne A，et al. Blood-Brain Barrier：from physiology to disease and back [J]. Physiol Rev，2019，99（1）：21-78.
[2] Wu LW，Yin F，Peng R，et al. The tight junction proteins ZO-1，occludin and actin participate in the permeability increasing ofblood brain barrier induced by hypoxia-ischemia [J]. Chin J Contemp Pediatr，2008，10（4）：513-516.
[3] 申杰，朱润秀，袁军，等.血小板源性生长因子受体-β对局灶性脑缺血小鼠血-脑屏障通透性的影响[J].中国脑血管病杂志，2016，13（11）：593-600.
[4] 陆定艳，李靖，孙佳，等.参芎葡萄糖注射液通过激活PI3K/AKT通路拮抗H2O2诱导H9c2细胞凋亡[J].中国中药杂志，2019，44（17）：3773-3779.
[5] 张留宝，陈进贵，刘平.不同动物的不同脑供血动脉结扎后存活率比较[J].南京军医学院学报，2002，24（2）：73-74.
[6] 姜恩平，王帅群，王卓，等.北五味子总木脂素对脑缺血模型大鼠神经细胞凋亡及p-AKT表达的影响[J].中国中药杂志，2014，39（9）：1680-1684.
[7] 胡丽，金红阳，陈娜，等.脑血流变化评价正常颅压脑积水诊治中脑脊液释放试验的意义[J].临床合理用药杂志，2021，14（15）：166-168.
[8] 王葳，陈桂英，王春雨，等.脑脊液与血清白蛋白比值变化对血脑屏障功能障碍评估的临床价值[J].中国医药指南，2015，13（20）：90-91.
[9] Chen RL，Chen CP，Preston JE. Elevation of CSF albumin in old sheep：relations to CSF turnover and albumin extraction at blood-CSF barrier [J]. J Neurochem，2010，113（5）：1230-1239.
[10] Jain SK，Paul-Satyaseela M，Lamichhane G，et al. Mycobacterium tuerculosis invasion and traversal across an in vitro human blood-brain barrier as a pathogenic mechanism for central nervous system tuberculosis [J]. J Infect Dis，2006，193（9）：1287-1295.
[11] 魏微，张微微，王娟，等.丁苯肽对慢性低灌注大鼠血-脑脊液屏障保护作用的研究[J].神经损伤与功能重建，2010，5（5）：335-337，354.
[12] 马炜秀，王志文，袁强，等.麝香乌龙丸对S1P诱导血管内皮细胞通透性的调控作用[J].疑难病杂志，2020，19（8）：839-844.
[13] 李花，胡康丽，刘旺华，等.健脾益气利水方对脑缺血大鼠脑水肿及其脑组织Occludin、Claudin-1、ZO-1蛋白表达的影响[J].中华中医药杂志，2019，34（11）：5424-5428.
[14] 林爱金，王洁琼，张留影，等.血-脑脊液屏障与阿尔茨海默病关系研究[J].脑与神经疾病杂志，2020，28（2）：124-126.
[15] 王蔚，钟霞，杜渊，等.蛭龙活血通瘀胶囊对模型大鼠脑出血后脑组织水肿及ZO-1、Occludin的影响[J].中国中医急症，2020，29（12）：2084-2086，2096.
[16] 王振华，刘云会，马腾，等.大鼠局灶性脑缺血再灌注早期血脑屏障通透性变化的研究[J].中国医科大学学报，2012，41（12）：1073-1076.
[17] Hawkins BT，Davis TP. The blood-brain barrier neurovascular unit in health and disease [J]. Pharmacol Rev，2005，57（2）：173-185.
[18] Lee JM，Park JM，Song MK，et al. The ameliorative effects of exercise on cognitive impairment and white matter injury from blood-brain barrier disruption induced by chronic cerebral hypoperfusion in adolescent rats [J]. Neurosci Lett，2017，638：83-89.
[19] Matter K，Balda MS. Functional analysis of tight junctions [J]. Methods，2003，30（3）：228-234.
[20] 唐冰镕，李花，刘旺华，等.加味四君子汤对脑缺血大鼠脑组织Occludin、ZO-1、Claudin-1蛋白及其mRNA表达的影响[J].中国实验方剂学杂志，2019，25（15）：57-63.
[21] 李思宇，王庆松.慢性脑低灌注对大鼠回肠黏膜Occludin及Claudin-2表达的影响[J].中国组织工程研究，2020，24（32）：5186-5191.
[22] Gu Z，Cui J，Brown S，et al. A highly specific inhibitor of matrix metalloproteinase-9 rescues laminin from proteolysis and neurons from apoptosis in transient focal cerebral ischemia [J]. J Neurosci，2005，25（27）：6401-6408.
[23] 李希，万溪，高晶晶，等.化浊解毒活血通络方对脑缺血再灌注损伤大鼠ZO-1和occludin表达的影响[J].环球中医药，2017，10（7）：796-801.
[24] 刘新秦，郑刚，苏鹏，等.铅暴露对体外血脑脊液屏障功能及ZO-1和闭合蛋白表达的影响[J].中国药理学与毒理学杂志，2017，31（6）：615-620.
[25] 黄文东，杨艳芳，高琦，等.丹参川芎嗪对离体大鼠心肌缺血/再灌注损伤的保护作用[J].中国医药科学，2019， 9（5）：9-13.
[26] 王艳，汪宁.川芎嗪对体外血脑屏障模型通透性变化的影响及其对紧密连接蛋白ocludin、ZO-1表达的影响[C]//中华中医药学会.中华中医药学会第十次中药实验药理学术会议论文集，2013：35-36.
[27] 孙瑞，于德水.抑制miR-429促进ZO-1、Occludin和Claudin-5蛋白表达改善血脊髓屏障通透性的体外实验研究[J].中国修复重建外科杂志，2020，34（9）：1163-1169.
京公网安备 11010502046598号