Abstract：Objective To study the effect of total flavnoids of fructus choerospondiatis (TFFC) on arsenic trioxide (ATO)-induced cardiomyocyte injury, and to explore its mechanism of action. Methods Cardiomyocytes were isolated and cultivated by neonatal rats and the morphology of cardiomyocytes at different time periods was observed under an inverted microscope. Cardiomyocyte injure models were use established by 6 μg/ml ATO, the experiment set up blank group, model group, and TFFC low, medium, and high concentration groups. The CCK-8 method was used to detect the survival rate of each group of cells, the activity of glutamic oxaloacetic transaminase (GOT)，creatine kinase (CK) and lactate dehydrogenase (LDH) in cell culture medium were determined by automatic bio-chemical instrument; the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in the cells were detected by kit method. The degree of apoptosis was observed by Hoechst 33342 fluorescence staining. Cell apoptosis rates were measured by flow cytomety. Results The cells cultured for 48 hours were basically all attached to the wall. Most of the cells stuck out the pseudopodia and were accompanied by spontaneous beating, which can meet the experimental requirements. The cell survival rate was reduced by 6 μg/ml ATO. Compared with the model group, the cell survival rate of each TFFC concentration group significantly increased, the vitality of LDH, CK, and GOT in the cell culture fluid was significantly reduced, the vitality of SOD, GSH-Px, and CAT in the cell were significantly increased, and the apoptosis rate was significantly reduced, and the differences were statistically significant (P ＜ 0.05). Conclusion TFFC can improve the state of ATO-induced myocardial injury, thereby protecting the myocardium damaged by ATO.
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