加权基因共表达网络分析筛胃癌相关分子

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摘要目的运用加权基因共表达网络分析（WGCNA）筛选出与胃癌发生、预后相关的分子，为胃癌的治疗和预后判断提供参考依据。方法从TCGA数据库中下载胃癌患者基因表达谱和临床数据，检索时间为建库至2020年11月14日。采用WGCNA方法对胃癌患者进行基因表达分析；筛选出核心靶基因并分析其功能，利用ONCOMINE和GEO数据库进行验证。结果共纳入359例胃癌患者。BLUE模块与胃癌发生、预后相关，CDC5L为核心靶基因，CDC5L与胃癌发生、预后相关。ONCOMINE数据库验证结果显示，癌组织中CDC5L表达量高于癌旁组织（P ＜ 0.01）；GEO数据库验证结果显示，低表达CDC5L患者预后更好。结论 CDC5L基因与胃癌发生和患者生存相关，可为胃癌发生、转移和治疗的研究提供参考依据。

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	肖又德1 郑永法2 戈伟1

关键词 ：胃癌, 癌症基因组图谱数据库, CDC5L, 加权基因共表达网络分析

Abstract：Objective To screen out molecules related to the occurrence and prognosis of gastric cancer through weighted gene co-expression network analysis (WGCNA), so as to provide a reference for the treatment and prognosis of gastric cancer. Methods The gene expression profile and clinical data of gastric cancer patients were downloaded from the TCGA database, retrieval time was from the establishment to November 14, 2020. WGCNA was used to analyze gene expression in gastric cancer patients. The core target genes were screened and their functions were analyzed, and validation was performed using ONCOMINE and GEO databases. Results A total of 359 patients with gastric cancer were included. The BLUE module was associated with the occurrence and prognosis, CDC5L was acted as the core gene, and CDC5L was correlated with the occurrence and prognosis of gastric cancer. ONCOMINE database validation results showed that the expression of CDC5L in cancer tissues was higher than that in adjacent tissues (P < 0.01). GEO database validation results showed that patients with low expression of CDC5L had better prognosis. Conclusion CDC5L is associated with the occurrence and prognosis in gastric cancer patients, and it can provide reference for the study of the occurrence, metastasis and treatment of gastric cancer.

Key words： Gastric cancer Cancer genome atlas database CDC5L；Weighted gene co-expression network analysis

基金资助:国家自然科学基金青年科学基金项目（81703035）。

通讯作者: 戈伟（1960.5-），男，博士，教授，硕士生导师；研究方向：肿瘤微环境与肿瘤放射治疗。

作者简介: 肖又德（1989.11-），男，硕士；研究方向：常见恶性肿瘤的综合治疗。

引用本文:

肖又德1 郑永法2 戈伟1. 加权基因共表达网络分析筛胃癌相关分子[J]. 中国医药导报, 2021, 18(17): 8-12，24.
XIAO Youde1 ZHENG Yongfa2 GE Wei1. Screening of gastric cancer related molecules based on weighted gene co-expression network analysis. 中国医药导报, 2021, 18(17): 8-12，24.

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https://www.yiyaodaobao.com.cn/CN/ 或 https://www.yiyaodaobao.com.cn/CN/Y2021/V18/I17/8

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