慢性乙型肝炎患者血清病毒载量与炎症因子、T淋巴细胞亚群及肝脏酶的关系

doi:10.20047/j.issn1673-72102023.25.19

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摘要目的研究慢性乙型肝炎患者血清乙型肝炎病毒（HBV）载量与炎症因子、外周血T淋巴细胞亚群及肝脏酶关系。方法收集2019年3月至2022年8月上海中医药大学附属普陀医院收治的85例慢性乙型肝炎患者，按照HBV DNA表达载量将其分为HBV DNA阴性组（29例），HBV DNA低载量组（28例）和HBV DNA高载量组（28例）。比较三组炎症因子、外周血T淋巴细胞亚群及肝脏酶水平的差异，并进一步进行相关性分析。结果 HBV DNA低、高载量组白细胞介素（IL）-6、IL-8、IL-10、肿瘤坏死因子（TNF）、CD8+百分率、CD3+绝对计数、CD8+绝对计数、谷丙转氨酶（GPT）、谷草转氨酶（GOT）均高于HBV DNA阴性组，CD4+百分率、CD4+绝对计数、CD4+/CD8+低于HBV DNA阴性组（P＜0.05）。HBV DNA高载量组IL-6、IL-8、IL-10、TNF、GPT、GOT水平及CD8+百分率均高于HBV DNA低载量组，CD4+百分率、CD3+绝对计数、CD4+绝对计数、CD8+绝对计数、CD4+/CD8+低于HBV DNA低载量组（P＜0.05）。相关性分析结果显示，慢性乙型肝炎患者IL-6、IL-10、TNF水平与GPT、GOT水平均呈正相关（r＞0，P＜0.05）。结论 HBV感染导致慢性乙型肝炎患者炎症因子和T淋巴细胞亚群及肝脏酶水平改变，部分炎症因子与肝脏酶具有相关性，可能促使机体出现免疫级联反应。

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	陈自喜*　　李锦澎*　　郑瑾　　相芬芬　　康向东▲　　吴蓉▲

关键词 ：慢性乙型肝炎, 炎症因子, T淋巴细胞亚群, 肝脏酶

Abstract：Objective To study the relationship between serum hepatitis B virus (HBV) load and inflammatory factors, peripheral blood T lymphocyte subsets, and liver enzymes in chronic hepatitis B patients. Methods A total of 85 patients with chronic hepatitis B admitted to Putuo Hospital, Shanghai University of Traditional Chinese Medicine from March 2019 to August 2022 were collected and divided into HBV DNA negative group (29 cases), HBV DNA low load group (28 cases), and HBV DNA high load group (28 cases) according to their HBV DNA expression load. The differences of inflammatory factors, peripheral blood T lymphocyte subsets, and liver enzyme levels among the three groups were compared, and the correlation was further analyzed. Results Interleukin (IL-6), IL-8, IL-10, tumor necrosis factor (TNF), percentage of CD8+, absolute count of CD3+, absolute count of CD8+, glutamic-pyruvic transaminase (GPT), and glutamic-oxaloacetic transaminase (GOT) in HBV DNA low and high load groups were higher than those in HBV DNA negative group, percentage of CD4+, absolute count of CD4+, CD4+/CD8+ were lower than those in HBV DNA negative group (P＜0.05). The levels of IL-6, IL-8, IL-10, TNF, GPT, GOT, and percentage of CD8+ in HBV DNA high load group were higher than those in HBV DNA low load group, and the percentage of CD4+, absolute count of CD3+, absolute count of CD4+, absolute count of CD8+, CD4+/CD8+ were lower than those in HBV DNA low load group (P＜0.05). Correlation analysis showed that the levels of IL-6, IL-10, and TNF were positively correlated with GPT and GOT in patients with chronic hepatitis B (r＞0, P＜0.05). Conclusion HBV infection causes changes in the levels of inflammatory factors, T lymphocyte subsets and liver enzymes in patients with chronic hepatitis B. Some inflammatory factors are correlated with liver enzymes, which may promote the immune cascade in the body.

Key words： Chronic hepatitis B Inflammatory factors T lymphocyte subsets Liver function enzyme

基金资助:国家自然科学基金青年科学基金资助项目（31800988）；
上海市卫生健康委员会卫生行业临床研究专项青年项目（20214Y0502）；
上海市“科技创新行动计划”自然科学基金面上项目（23ZR1456400）。

通讯作者: ▲共同通讯作者

作者简介: 陈自喜（1989.5-），女，硕士；研究方向：分子生物学。 *具有同等贡献

引用本文:

陈自喜*　　李锦澎*　　郑瑾　　相芬芬　　康向东▲　　吴蓉▲. 慢性乙型肝炎患者血清病毒载量与炎症因子、T淋巴细胞亚群及肝脏酶的关系[J]. 中国医药导报, 2023, 20(25): 90-93.
CHEN Zixi* LI Jinpeng* ZHENG Jin XIANG Fenfen KANG Xiangdong▲ WU Rong▲. Relationship between serum viral load and inflammatory factors, T lymphocyte subsets, and liver enzymes in patients with chronic hepatitis B. 中国医药导报, 2023, 20(25): 90-93.

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