Abstract：Objective To investigate the effect of Yiqi Huoxue Shengji Prescription on ulcer wound healing and vessel regeneration in diabetic mellitus rats. Methods Sixty male SD rats with SPF grade 281-320 g and age of eight-ten weeks were divided into drug administration group, refractory wound group, normal wound group, and Sijunzi Decoction group according to random number table method, with 15 rats in each group. Among them, the drug administration group, refractory wound group, and Sijunzi Decoction group were prepared into diabetes mellitus model by streptozutocin method, fasting blood glucose ＞16.7 mmol/L after 48 h, with the occurrence of polyuria and polydipsia, indicating successful modeling. Skin defect wounds were made on the back of all rats. The drug administration group and Sijunzi Decoction group were given 3 ml Yiqi Huoxue Shengji Prescription and Sijunzi Decoction by gavage, respectively, and the other groups were given 3 ml double steaming water by gavage, once in the morning and once in the evening. On the 5th, 10th, and 20th day after surgery, five rats in each group were selected to measure the wound size and calculate the wound healing rate. Hematoxylin-eosin staining was used to observe and calculate the number of capillaries on the ulcer wound. The content of vascular endothelial growth factor (VEGF) in the blood of rats was detected by enzyme-linked immunoadsordent assay. The content of VEGF, transforming growth factor-β1 (TGF-β1), and Smad3 in the local tissue of the wound was detected by Western blot. Results Wound healing rate, serum VEGF expression level, and VEGF protein expression level in local tissue of wound in each group on the 20th day after surgery were higher than those on the 5th and 10th day after surgery; wound healing rate, serum VEGF expression level, and VEGF protein expression level in local tissue of wound in each group on the 10th day after surgery were higher than those on the 5th day after surgery (P＜0.05). The protein expression levels of TGF-β1 and Smad3 in drug administration group and normal wound group on the 20th day after surgery were lower than those in this group on the 5th and 10th day after surgery, and the protein expression levels of TGF-β1 and Smad3 in drug administration group and normal wound group on the 10th day after surgery were lower than those in this group on the 5th day after surgery (P＜0.05). The protein expression levels of TGF-β1 in refractory wound group on the 20th day after surgery were lower than those on the 5th and 10th day after surgery (P＜0.05). The expression level of Smad3 protein in refractory wound group on the 10th and 20th day after surgery were higher than those on the 5th day after surgery (P＜0.05). On the 5th day after surgery, the wound healing rate of refractory wound group was lower than that of normal wound group (P＜0.05). On the 10th and 20th day after surgery, the wound healing rate in the refractory wound group was lower than that in the normal wound group, and the wound healing rate in the drug administration group was higher than that in the refractory wound group (P＜0.05). The serum VEGF expression level and VEGF protein expression level in local wound tissue of refractory wound group were lower than those of normal wound group at all time points after surgery, and the serum VEGF expression level and VEGF protein expression level in local wound tissue of drug administration group were higher than those of refractory wound group (P＜0.05). On the 5th and 10th day after surgery, the protein expression levels of TGF-β1 and Smad3 in refractory wound group were lower than those in normal wound group, and the protein expression levels of TGF-β1 and Smad3 in drug administration group were higher than those in refractory wound group (P＜0.05). On the 20th day after surgery, the protein expression levels of TGF-β1 and Smad3 in refractory wound group were higher than those in normal wound group, while the protein expression levels of TGF-β1 and Smad3 in drug administration group were lower than those in refractory wound group (P＜0.05). Pathological observation showed that the inflammatory cell infiltration and tissue necrosis in the drug administration group were lighter than those in the refractory wound group, and the formation of capillaries was obvious. Conclusion Yiqi Huoxue Shengji Prescription can promote the secretion of VEGF in diabetic rats, regulate the expression of TGF-β1 and Smad3 proteins, promote neovascularization, and then promote the healing of refractory wounds.
骆渊城 朱滨 杨威 王奎. 益气活血生肌方对难愈性创面愈合及血管再生的影响[J]. 中国医药导报, 2023, 20(12): 30-34.
LUO Yuancheng ZHU Bin YANG Wei WANG Kui. Effect of Yiqi Huoxue Shengji Prescription on refractory wound healing and vessel regeneration. 中国医药导报, 2023, 20(12): 30-34.
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