|
|
|
| Effect of Zhuanggu Zhitong prescription on CGRP and β2-AR in ovariectomized osteoporosis rats |
| GAN Guoxing1 LIU Yu1 LI Jinping2 ZHANG Guomin3 YI Zhongyuan1 |
1.Ministry of Science and Education, Qingyuan Hospital of Traditional Chinese Medicine, Guangdong Province, Qingyuan 511500, China;
2.Xiangya School of Pharmacy, Central South University, Hu′nan Province, Changsha 410013, China;
3. Basic Medical College, Hunan University of Traditional Chinese Medicine, Hu′nan Province, Changsha 410028, China |
|
|
|
Abstract Objective To explore the effect of Zhuanggu Zhitong prescription on the CGRP and β2-AR in ovariectomized osteoporosis rats and investigate its mechanism in anti-osteoporosis. Method 72 rats were randomly divided into sham operation group (12 cases) and model group (60 cases), the model group rats were ovariectomized osteoporosis and the sham operation group rats were excised equal weight fat. After the modeling, the rats were randomly divided into model group, Zhuanggu Zhitong prescription high-dose group (13.2 g/kg), middle-dose group (6.6 g/kg), low-dose group (3.3 g/kg) and estradiol valerate group (2.1×10-5 g/kg), with 12 rats in each group. The model group and sham operation group rats were given equal volume water. All intervened for 13 weeks. The content of CGRP in serum was detected by ELISA and protein expression of β2-AR in bone tissue were detected by immunohistochemistry. Results Compared with sham operation group, the content of CGRP in serum and protein expression of β2-AR in bone tissue of model group were significantly declined, with high statistically significant differences (all P ＜ 0.01). Compared with the model group, the content of CGRP in serum and protein expression of β2-AR in bone tissue of different doses Zhuanggu Zhitong prescription group and estradiol valerate group were increased significantly, with statistically significant differences (P ＜ 0.05 or P ＜ 0.01). There were no significant differences in different doses of Zhuanggu Zhitong prescription groups (P ＞ 0.05). Conclusion Zhuanggu Zhitong prescription can promote the secretion of CGRP and protein expression of β2-AR, which may be one mechanism of ZhuangguZhitong prescription to anti-osteoporosis.
|
|
|
|
|
|
[1] 曾英，莫新民，雷晓明，等.壮骨止痛胶囊对去卵巢雌鼠骨质疏松症骨密度及相关生化指标的影响[J].湖南中医药大学学报，2008，28（2）：10-12.
[2] 李应福，李宁，谢兴文.OPG/RANK/RANKL信号轴与原发性骨质疏松关系的研究进展[J].中国骨质疏松杂志，2016，22（1）：115-119.
[3] 甘国兴，李劲平，刘毓，等.壮骨止痛方调节OPG/RANKL平衡抗绝经后骨质疏松作用[J].中国现代应用药学，2017， 34（7）：938-942.
[4] 王杨雨凡，陈一心.交感神经系统对骨量调控作用的研究进展[J].中国骨质疏松杂志，2012，18（9）：854-860.
[5] 郭纪涛，谭雄进.降钙素基因相关肽对骨质疏松症骨组织及胃肠作用的研究进展[J].中国骨质疏松杂志，2015， 21（1）：121-124.
[6] 梁伟.降钙素基因相关肽对骨质疏松大鼠骨髓基质干细胞成骨分化的影响[D].南宁：广西医科大学，2014.
[7] 杨亚东，周娟，高辉，等.失神经支配在大鼠骨折愈合过程中降钙素基因相关肽对骨保护素/破骨细胞分化因子影响的实验研究[J].中华手外科杂志，2012，28（4）：233-237.
[8] 陈光华，黄贵芝，林颢，等.降钙素基因相关肽对维甲酸致老年骨质疏松大鼠OPG/RANKL信号通路的影响及疗效[J].中国老年学杂志，2015，35（19）：5446-5448.
[9] 朱晓峰，王廷春，张荣华，等.益骨胶囊对去卵巢大鼠骨密度及血浆和骨组织CGRP和SP含量的影响[J].时珍国医国药，2012，23（5）：1054-1056.
[10] 吕辰鹏，杨丽，孙影，等.去卵巢骨质疏松模型大鼠骨组织CGRP及其1型受体的表达[J].中国病理生理杂志，2011，27（5）：976-979.
[11] Zaidi M. Neural surveillance of skeletal homeostasis [J]. Cell Metab，2005，1（4）：219-221.
[12] Nagao M，Feinstein TN，Ezura Y，et al. Sympathetic control of bone mass regulated by osteopontin [J]. Proc Natl Acad Sci USA，2011，108（43）：17 767-17 772.
[13] He JY，Jiang LS，Dai LY. The roles of the sympathetic nervous system in osteoporotic diseases：A review of experimental and clinical studies [J]. Ageing Res Rev，2011， 10（2）：253-263.
[14] Kajimura D，Hinoi E，Ferron M，et al. Genetic determination of the cellular basis of the sympathetic regulation of bone mass accrual [J]. J Exp Med，2011，208（4）：841-851.
[15] Elefteriou F，Ahn JD，Takeda S，et al. Leptin regulation of bone resorption by the sympathetic nervous system and CART [J]. Nature，2005，434（7032）：514-520.
[16] 周正，赵长铭，焦凯，等.交感神经系统-肾上腺素能受体对骨改建的调节作用[J].国际口腔医学杂志，2015， 42（3）：348-351.
[17] Komoto S，Kondo H，Fukuta O，et al. Comparison of β-adrenergic and glucocorticoid signaling on clock gene and osteoblast-related gene expressions in human osteoblast [J]. Chronobiol Int，2012，29（1）：66-74.
[18] Li H，Fong C，Chen Y，et al. Beta2- and beta3-，but notbeta1-adrenergic receptors are involved in osteogenesis of mouse mesenchymal stem cells via cAMP/PKA signaling [J]. Arch Biochem Biophys，2010，496（2）：77-83.
[19] 孙影，杨丽，吕辰鹏，等.骨碎补总黄酮对去卵巢骨质疏松模型大鼠血清中瘦素、白细胞介素6、前列腺素E2及骨组织中β2-肾上腺素受体的影响[J].中国病理生理杂志，2011，27（4）：755-758.
[20] 杨军，莫新民.壮骨止痛方对骨质疏松大鼠Wnt β-catenin、RANKL/RANK/OPG信号通路的影响[J].北京中医药，2017，36（7）：611-613. |
|
|
|
