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| The content difference of gastrodin between Gastrodia Rhizoma prepared by ginger from Jianchang Faction and other processed products in Jiangxi Province |
| LU Ping1 JIN Lei1 JIA Caihong1 LI Xuran2 NIAN Fenghong2 GONG Qianfeng2 YE Xide2 |
1.Department of Pharmacy, the 105th Hospital of Chinese People′s Liberation Army, Anhui Province, Hefei 230031, China;
2.College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Jiangxi Province, Nanchang 330004, China |
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Abstract Objective To establish a method for detecting the contents of gastrodin in processed products of Gastrodia Rhizoma, and to compare the content difference of gastrodin between Gastrodia Rhizoma prepared by ginger and other processed products. Methods High performance liquid chromatography method was conducted for the analysis. The chromatographic column was Ultimate3000 C18 (4.6 mm×250 mm, 5 μm), the flowing phase was acetonitrile-water (3∶97), the detection wavelength was 220 nm, the flow rate was 1 mL/min, and the column temperature was 25℃. Results The sample amount and peak area of gastrodin showed good linear relationship, the average recovery rate was 99.76%, RSD was 1.42%, the contents of gastrodin in Gastrodia Rhizoma prepared by ginger, raw Gastrodia Rhizoma and Gastrodia Rhizoma prepared by sulfur fumigation were 1.001%, 0.458%, 0.406% respectively. Conclusion The contents of gastrodin in Gastrodia Rhizoma prepared by ginger shows significant differences with other processed products. This method is sensitive, fast and easy, which can provide reference for further research of Gastrodia Rhizoma.
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