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| Effects of Pholidota chinensis Lindl Polysaccharides on the levels of chemokines of bronchoalveolar lavage fluid in a murine model of Mycoplasma pneumoniae pneumonia |
| DU Huichao1 HUANG Meiqing2 ZHAO Shiyuan3 |
1.Department of Pharmacy, Chongzuo People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Zhuang Autonomous Region, Chongzuo 532200, China;
2.Department of Paediatrics, Chongzuo People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Zhuang Autonomous Region, Chongzuo 532200, China;
3.Affiliated National Medical Center Laboratory of Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning 530001, China |
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Abstract Objective To investigate the Pholidota chinensis Lindl Polysaccharides (PCLP) on mice model of Mycoplasma pneumoniae pneumonia in bronchoalveolar lavage fluid (BALF) effect of chemokine. Methods Ninety BABL/C nude mice were randomized into model group, clarithromycin group and polysaccharide group, 30 rats in each group. Medium of Mycoplasma pneumoniae with PPLO broth, the successful isolation of Mycoplasma pneumoniae drops into the nasal cavity in 1×107 CCU/mL 50 μL, 8 d, on the second day after inoculation to 1 last polysaccharide with Pholidota intervention. After intervention of 1, 3 d and 6 d, respectively, bronchoalveolar lavage fluid (BALF) were collected, Mycoplasma pneumoniae of BALF was cutured by SP4 agar plate quantitative recovery of lung tissue, by formalin fixation and HE staining, the lesions of mouse lung of Mycoplasma pneumoniae infection in BALF mice were observed, Macrophage inflammatory protein-1α (MIP-1α), MIG, KC, Macrophage inflammatory protein-1 (MCP-1) and interferon-γ-inducible protein 10 (IP-10) chemotaxis cytokine levels were measured by ELISA. Results BAL Mycoplasma pneumoniae pneumonia concentrations in mice treated with PCLP tended to be reduced compared with mice treated with placebo (MpPl), however there was no statistically significant difference (P > 0.05); the lung HPS was significantly lower, as well as the parenchymal-pneumonia subscore. 3 d after Polysaccharide intervention in Mycoplasma pneumoniae pneumonia mice, the expression of chemokines MIP-1α, MIG and IP-10 in BALF mice were significantly reduced, the differences were statistically significant (P < 0.05). Conclusion Polysaccharide significantly reduce the Mycoplasma pneumoniae lung inflammation in mice, the mechanism may be related to the inhibition of MIP-1α, MIG and IP-10 chemokines in vivo in mice model of Mycoplasma pneumoniae.
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