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| The diagnostic value of neutrophil CD64 and monocyte HLA-DR in bacterial and viral infections |
| YU Jianhua1 ZHANG Yanhong1 ZOU Xiaofeng1 ZHU Wenting1 LI Xin2 CHEN Meiqi3 |
1.Department of Central Hematologic Laboratory, Dongguan Kanghua Hospital, Guangdong Province, Dongguan 532080, China;
2.Department of Oncology, Dongguan Kanghua Hospital, Guangdong Province, Dongguan 532080, China;
3.School of Basic Medical Sciences, Southern Medical University, Guangdong Province, Guangzhou 510515, China |
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Abstract Objective To explore the diagnostic value of neutrophil CD64 index (nCD64 index) and monocyte human leukocyte antigen DR (mHLA-DR) in distinguishing bacterial and viral infections. Methods A total of 74 fever patients diagnosed and treated by Dongguan Kanghua Hospital (hereinafter referred to as “our hospital”) were selected from January to November 2019. According to the cause, they were divided into bacterial infection group (40 cases) and viral infection group (34 cases), while 40 healthy people who received physical examination in the hospital during the same period were served as healthy controls. The levels of CD64 and HLA-DR in each group were detected and compared, and receiver operating curve (ROC) was used to analyze the diagnostic value of each index in identifying infections. Results The nCD64 and nCD64 index of the bacterial infection group were significantly higher than those of the healthy control group and the virus infection group, and the differences were statistically significant (P < 0.05); the virus infection group was positive for mHLA-DR and the percentage of monocyte HLA-DR [mHLA-DR+(%)] were significantly higher than those of the healthy control group and the bacterial infection group, and the differences were statistically significant (P < 0.05). The best cut-off values of nCD64, nCD64 index, mHLA-DR and mHLA-DR+ (%) in identifying infections were 30.8, 0.53, 325 and 93.9%, respectively, while the area under the ROC were 0.929, 0.969, 0.778 and 0.782, respectively. The combined detection of nCD64 index and mHLA-DR had an area under the differential infection curve of 0.971, with high sensitivity (95.00%) and specificity (94.12%). Conclusion The combined detection of nCD64 index and mHLA-DR by flow cytometry can be used for the differential diagnosis of bacterial and viral infections.
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