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| Effects of flavonoids extracted from Alternanthera philoxeroides on HBsAg and HBeAg expression content in HepG2. 2. 15 cells |
| WU Qian-hu1 XU Weidong2 LI Weidong3 XU Lu4 LING Mei1 WU Yuhua1 |
1.Drug Clinical Trial Institution Office, Zhenjiang Third Hospital Affiliated to Jiangsu University, Jiangsu Province, Zhenjiang 212000, China;
2.School of Pharmacy, Jiangsu University, Jiangsu Province, Zhenjiang 212013, China;
3.School of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210023, China |
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Abstract Objective To study the anti-HBV action of total flavonoids extracted from Alternanthera philoxeroides in vitro. Methods HepG2.2.15 cells were inoculated into the cell culture plates and then randomly divided into negative control group, experimental group and positive control group. The negative control group was treated with 100 mL/L DMSO, the experimental groups were treated with 800, 400, 200 and 100 mg/L total flavonoids extracted from Alternanthera philoxeroides. In the positive control groups, lamivudine was added at the concentration of 800, 400, 200 and 100 mg/L. Cell culture and optical density assay were used to study the effects of total flavonoids from Alternanthera philoxeroides on the expression of HBsAg and HBeAg in HepG2.2.15 cell line. Results The inhibit the expression of HBsAg in HepG2.2.15 cells in each experimental group was obviously higher, compared with the negative group, the difference was statistically significant (P < 0.01) and compared with the positive control group, the difference was statistically significant (P < 0.05), the inhibition rate of HBsAg in the experimental group was positively correlated with the dose of the drug. Each dose of the experimental groups had obvious inhibitory effects on the expressions of HBeAg in HepG2.2.15 cells, compared with the negative control group, the difference was statistically significant (P < 0.01) compared with and positive control group, the difference was statistically significant (P < 0.05), the inhibition rate of HBeAg in the experimental group was positively correlated with the dose of the drug. Conclusion Total flavonoids extracted from Alternanthera philoxeroides has obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2. 2. 15 cells. Total flavonoids extracted from Alternanthera philoxeroides has strong anti-HBV action in vitro.
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[1] 武谦虎，樊一桥，洪敏，等.空心莲子草醇提物对小鼠免疫性肝损伤的保护作用[J].中国医院药学杂志，2006，26（4）：373-375.
[2] 武谦虎，樊一桥，洪敏，等.空心莲子草醇提物对化学性肝损伤的影响[J].南京中医药大学学报：自然科学版，2006，22（1）：38-41.
[3] 阎纳新，武谦虎. 空心莲子草醇提物对小鼠急性酒精性肝损伤的防护作用[J].中国药业，2015，24（14）：26-28.
[4] 吴翠松.空心莲子草治疗慢性乙肝的临床疗效观察[J].医学信息旬刊，2011，24（10）：6585.
[5] 方进波，刘焱文，张彦文，等.空心莲子草抗病毒活性成分研究[J].中草药，2007，38（7）：976.
[6] 王岩.空心莲子草正丁醇部位免疫调节作用研究[J].中国民族民间医药，2011，20（5）：36.
[7] 徐庆，陈全斌，义祥辉，等.荔枝核提取物对HepG2.2.15细胞系HBsAg与HBeAg表达的影响[J].中国医院药学杂志，2004，24（7）：393-395.
[8] 王青青，余海.SRB显色法用于抗癌药物敏感性试验的研究[J].科技通报，2000，16（2）：104-107.
[9] 师磊.空心莲子草乙酸乙酯提取物抗病毒活性成分及质量分析研究[D].武汉：湖北中医学院，2008.
[10] 李森.空心莲子草抗病毒有效部位群提取物制备工艺及其成分分析研究[D].武汉：湖北中医药大学，2012.
[11] 廖鹏程.空心莲子草有效部位冻干粉针剂的药学研究[D].武汉：湖北中医药大学，2013.
[12] 李爽.空心莲子草有效部位群提取物抗病毒活性及其成分分析研究[D].武汉：湖北中医药大学，2013.
[13] 蒋文玲，杨占秋，陈文，等.空心莲子草有效成分抗呼吸道合胞病毒作用的实验研究[J].广州：南方医科大学学报，2007，27（1）：62-64.
[14] 李爽，彭晓芝，廖鹏程，等.空心莲子草有效部位提取物抗甲型H3N2流感病毒作用[J].天津中医药，2012，29（5）：478-480.
[15] 张周菊.空心莲子草抗病毒有效部位群提取物的质量评价体系研究[D].武汉：湖北中医药大学，2012.
[16] 王杰，鲁凤民.再认识乙肝病毒实现临床“治愈”[J].中华医学信息导报，2017，32（1）：20.
[17] 王艳.HepG2.2.15细胞脂肪变性对HBV基因表达及SOCS-3和SREBP-1c通路的影响[D].太原：山西医科大学，2011.
[18] 边中启，刘霜，刘明秋，等.靶向preC/C基因特异性RNA干扰对乙型肝炎病毒在肝癌细胞系中复制与表达的影响[J].中华医学杂志，2012，92（11）：768-772.
[19] 朱锦宏，王春苗，吴红，等.乙型肝炎病毒X基因siRNA对HepG2.2.15细胞的影响[J].实用医学杂志，2016，32（13）：2120-2123.
[20] 杨伟，赵超，李婧，等.恩替卡韦和干扰素序贯作用对HepG2.2.15细胞HBV DNA复制的影响[J].胃肠病学和肝病学杂志，2016，25（4）：408-410.
[21] Jing-yao PANG，Kui-jun ZHAO，Jia-bo WANG，et al. Green tea polyphenol, epigallocatechin-3-gallate, possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro[J].Biomedicine & Biotechnology，2014，（6）：533-539. |
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