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| Effects of Butylphthalide on the expression of GSK-3β and p-Tau proteins in CA1 region of hippocampal of Alzheimer′s disease rats |
| DENG Chunying1 LI haibin2 MAO Wenjing1 LI Shiying1 |
1.Department of Neurology, North China University of Science and Technology Affiliated Hospital, Hebei Province, Tangshan 063000, China;
2.Department of Cardiology, People′s Hospital of Zunhua, Hebei Province, Zunhua 064200, China |
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Abstract Objetive To observe the effects of Butylphthalide (NBP) on the expression of glycogen synthase kinase-3β (GSK-3β) protein and p-Tau protein in CA1 region of hippocampus of Alzheimer′s disease rats, and to explore the mechanism of brain protection by butylphthalide. Method Seventy-two male SD rats were randomly divided into sham operation group (24 rats), AD model group (24 rats) and NBP treatment group (24 rats). Aβ-amyloid 1-42 (Aβ1-42) was used to make AD model. The sham operation group was injected with 5 μL saline. Four weeks after successful modeling, NBP treatment group was given NBP mixed solution made of NBP and edible oil by gavage. The sham operation group and the AD model group were given the same dose of edible oil. Morris water maze experiment was used to compare the number of times the rats crossed the platform. Western blot was used to detect the expression of GSK-3β and p-Tau proteins in the hippocampal CA1 region at one, two, four and eight weeks after administration. Results Compared with the sham operation group, the number of rat crossing the platform at each time point in AD model group was significantly reduced (all P ＜ 0.05). Compared with AD model group, the number of rats crossing the platform increased at each time point in the NBP treatment group (all P ＜ 0.05). Compared with the sham operation group in the same period, the relative expression levels of p-Tau and GSK-3β proteins in the hippocampal CA1 area of the AD model group were increased, and the differences were highly statistically significant (P ＜ 0.01). Compared with the AD model group in the same period, the relative expression levels of p-Tau and GSK-3β proteins in the hippocampal CA1 area of rats in the NBP treatment group were decreased, and the differences were highly statistically significant (P ＜ 0.01). Compared with the sham operation group in the same period, the relative expression levels of p-Tau and GSK-3β proteins in the hippocampal CA1 area of rats in the NBP treatment group were increased, and the difference was statistically significant (P ＜ 0.01). Conclusion NBP can play a protective role on the brain of AD model rats by down-regulating the expression of GSK-3β and p-Tau protein.
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