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| Effect of flavones derived from Saussurea medusa Maxim. for the HaCaT cells injury caused by UVB radiation |
| WENG Tengyu1 GUO Yan2 |
1.Department of Dermatology, Affiliated Hospital of Qinghai University, Qinghai Province, Xining 810000, China;
2.Department of Medical Cosmetology, Affiliated Hospital of Qinghai University, Qinghai Province, Xining 810000, China |
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Abstract Objective To investigate the effects of flavones derived from Saussurea medusa Maxim. for the HaCaT cells injury caused by UVB radiation. Methods The HaCaT cells line cultured in vitro was randomly divided into two groups, which were respectively given UVB irradiation at doses of 25 mJ/cm2 and 50 mJ/cm2. Then, the cells were further subdivided into four groups, including control group, UVB model group, 2 g/L of flavones derived from Saussurea medusa Maxim. group, and 8 g/L of flavones derived from Saussurea medusa Maxim. group. The cell proliferation was determined by MTT assay. The activities of superoxide dismutase (SOD), contents of glutathione (GSH), malondialdehyde (MDA) and activities of catalase (CAT) were detected by enzyme linked immunosorbent assay. Results In different doses of UVB radiation groups, compared with control group, the absorbance of UVB model group was decreased, the activities of SOD, CAT and the contents of GSH were reduced, while the contents of MDA were increased, the differences were all statistically significant (P ＜ 0.05). Compared with UVB model group, the absorbance of flavones derived from Saussurea medusa Maxim. group was increased, the activities of SOD, CAT and the contents of GSH were enhanced, while the contents of MDA were decreased, the differences were all statistically significant (P ＜ 0.05). Conclusion The flavones derived from Saussurea medusa Maxim. have certain protective effects for the oxidative damage of HaCaT cells after UVB radiation
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