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| Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage |
| WANG Rui1 CHEN Guimei1 LI Wei1 LIU Lanying1▲ LI Jiaoyan1 LI Genmao2 GE Dongyu2 WANG Yaoxian3 |
1.Department of Nephrology, the Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China;
2.Basic Research Center, Beijing University of Chinese Medicine, Beijing 100029, China;
3.Department of Nephrology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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Abstract Objective To investigate the effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2 (MMP-2), tssue inhibitor of metalloproteinase-2 (TIMP-2) of nephridial tissue in rats with ischemic nephropathy of advanced stage. Methods Male SD rats were randomly divided into sham operation group, model group (both groups were given normal saline 3 mL/d), drug group [Sanjie Tongluo Decoction 10.85 g/(kg·d)] by random number table method. The unilateral renal artery insufficiency ligation was used to establish animal model. In the 28th day of experiment, the levels of 24 hours urinary protein and creatinine of rats were detected. The rats were put to death and nephridial tissue was collected to take light microscope examination, the changes of renal morphology in all groups were observed. Immunohistochemistry was used to detect the expression of MMP-2 and TIMP-2 of nephridial tissue. Results Under the light microscope, the glomerulus and tubulointerstitium in the model group and drug group had damage. Compared with sham operation group, the scores of glomerulosclerotic index and tubulointerstitial lesion in the model group and drug group were all increased (P ＜ 0.01), which of drug group were lower than those of model group (P ＜ 0.01). Compared with model group, the expression of MMP-2 in drug group was increased (P ＜ 0.01), 24 hours urinary protein, creatinine and the expression of TIMP-2 were decreased (P ＜ 0.01), which of both groups were higher than those of sham operation group (P ＜ 0.01). Conclusion Sanjie Tongluo Decoction can prevent the lesions of ischemic nephropathy of advanced stage by up-regulating the expression of MMP2 and down-regulating the expression of TIMP-2.
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| Cite this article: |
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WANG Rui1 CHEN Guimei1 LI Wei1 LIU Lanying1▲ LI Jiaoyan1 LI Genmao2 GE Dongyu2 WANG Yaoxian3. Effects of Sanjie Tongluo Decoction on the expression of matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 2 in rats with ischemic nephropathy of advanced stage[J]. 中国医药导报, 2017, 14(29): 24-27.
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| URL: |
| http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I29/24 |
[1] 刘慧兰.缺血性肾病的诊断与治疗[J].中国全科医学，2006，9（2）：95-96.
[2] 陈桂梅.“散结通络方”干预慢性缺血性肾病的临床观察及动物实验研究[D].北京：北京中医药大学，2016.
[3] 毕增祺，郑法雷.慢性缺血性肾病的临床表现与主要病理生理改变[J].中华内科杂志，2000，39（6）：418-419.
[4] Radford MG Jr，Donadio JV Jr，Bergstralh EJ，et al. Predicting renal outcome in IgA nephropathy [J]. J AM Soc Nephrol，1997，8（2）：199-207.
[5] 黄悦.益气活血通络法治疗早期IRD的临床研究[D].北京：北京中医药大学，2012.
[6] 李伟，刘兰英，李娇艳，等.益气通络方对慢性缺血性肾病早期大鼠AngⅡ、α-SMA表达的影响[J].中国中医基础医学杂志，2014，20（1）：52-53.
[7] 吴以岭.脉络论[M].北京：中国科学技术出版社，2010：184-186.
[8] LIU Y. Cellular and molecular mechanisms of renal fibrosis [J]. Nat Rev Nephrol，2011，7（12）：684-696.
[9] 贺玉珍，张李梅，牟授菡，等.褐藻多糖硫酸酯对TGF-β1孵育的人肾小球系膜细胞MMP-2、TIMP-2表达的影响[J].滨州医学院学报，2012，35（2）：104-107.
[10] 曾海飞，何泽云，徐琴，等.六味地黄汤对5/6肾切除大鼠MMP-2，TIMP-2表达的影响[J].湖南中医药大学学报，2013，33（7）：13-17.
[11] 郭碧林，朱春玲.Egr-1蛋白、MMP-2、TGF-β1在肾小管间质纤维化大鼠中的表达及意义[J].中国现代医学杂志，2013，23（7）：27-33.
[12] 陈荣权，陈香美，崔世维，等.基质金属蛋白酶/组织金属蛋白酶抑制物表达失衡在衰老大鼠肾小管间质损害中的意义[J].中华医学杂志，2014，84（11）：58-63.
[13] 陆迅，魏明刚.黄芪多糖对肾间质纤维化大鼠的保护作用[J].中华中医药杂志，2014，29（6）：1998-2001.
[14] 马秀娜.阿托法他汀对高血压肾病患者基质金属蛋白酶-2和尿微量白蛋白/肌酐的影响[J].医疗装备，2016， 29（13）：94.
[15] 陈文军，袁国栋，檀金川.红花黄色素注射液对膜性肾病大鼠的肾保护作用及对基质金属蛋白酶抑制物-1、基质金属蛋白酶-9表达的影响[J].中药药理与临床，2016，32（3）：65-68.
[16] 刘红霞，张昱，李澎，等.加味黄芪赤风汤含药血清对LPS诱导的小鼠肾小球系膜细胞Col-Ⅳ、MMP-2及TIMP-2表达的影响[J].中国中西医结合杂志，2016，36（5）：592-596.
[17] 杨秀，马立彬，谢祥成，等.大黄素对糖尿病大鼠肾组织基质金属蛋白酶-２及金属蛋白酶组织抑制剂-２表达的影响[J].中国糖尿病杂志，2016，24（2）：159-162.
[18] 曲萌，董佳婧，姜锐.红参发酵产物对高糖下大鼠肾小球系膜细胞增殖和细胞外基质降解的影响[J].吉林大学学报：医学版，2017，43（2）：245-249.
[19] 董喜环.血清NGAL、CysC、RBP及MMP-2联合检测对糖尿病肾病的诊断价值[J].国际检验医学杂志，2016， 37（14）：1965-1967.
[20] 童晔玲，任泽明，陈璇，等.三七总皂苷对TNF-α诱导的肾小球系膜细胞增殖及MMPs/TIMPs表达的影响[J].中国现代应用药学，2017，34（6）：820-823. |
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