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| Analysis of UBQLN-1 expression and clinical significance in rectal cancer tissues and correlation with prognosis |
| SUN Guangyuan1 WU Xueliang1 XUE Jun1 ZHANG Haiyan2 MA Haiyan1 LIU Xiaoning3 HAN Lei1 QU Ming1 |
1.Department of General Surgery, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
2.Department of Neurology, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
3.Intensive Care Unit, 81th Army Hospital of the Army, Hebei Province, Zhangjiakou 075000, China |
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Abstract Objective To explore the expression and clinical significance of ubiquitin-1 (UBQLN-1) in rectal cancer, and correlation analysis with prognosis . Methods From December 2015 to January 2016, a total of 90 patients who underwent rectal cancer surgery in the Department of General Surgery of the First Affiliated Hospital Hebei North University were selected. The rectal cancer foci and normal tissues adjacent to the cancer were collected. Reverse transcription polymerase chain reaction (Rt-PCR) was used. The expression of UBQLN-1 mRNA of specimens in the two groups were detected, while immunohistochemistry was used to detect the expression of UBQLN-1 protein of specimens in the two groups, and the relationship with the clinicopathological characteristics of the tumor was analyzed. The survival period of the included patients was followed up, Kaplan-Meier was used to draw survival curves, and the relationship between the expression of UBQLN-1 protein and the prognosis of rectal cancer patients were analyzed by multi-factor prognosis. Results The relative expression of UBQLN-1 mRNA in rectal cancer tissues was significantly higher than that in normal tissues adjacent to cancer, and the difference was highly statistically significant (P < 0.01). The positive expression of UBQLN-1 in rectal cancer tissues was significantly higher than that in normal tissues adjacent to cancer, and the difference was highly statistically significant (P < 0.01). The positive expression of UBQLN-1 in rectal cancer tissues of patients with different levels of differentiation, T stage, lymph node metastasis and liver metastasis were compared, and the differences were statistically significant (P ＜ 0.05). The 5-year disease-free survival rate of rectal cancer patients with positive expression of UBQLN-1 was significantly lower than that of patients with negative expression, and the difference was statistically significant (P ＜ 0.05). T staging (HR = 1.246), lymph node metastasis (HR = 2.538), liver metastasis (HR = 2.256) and UBQLN-1 expression (HR = 5.820) were independent risk factors that affected the prognosis of patients (P ＜ 0.05). Conclusion The high expression of UBQLN-1 in rectal cancer tissue is closely related to the occurrence, development, invasion and metastasis of rectal cancer. It is also an independent risk factor for the prognosis of rectal cancer patients. Therefore, it can be used as a potential molecular biological indicator for early diagnosis and prognosis evaluation of rectal cancer.
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