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| Effect of hydrogen inhalation on lung injury induced by severe acute pancreatitis in rats |
| TENG Na SUN Mingjie LI Hui▲ |
| Department of Anesthesia Operating, Qingdao Municipal Hospital, Shandong Province, Qingdao 266011, China |
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Abstract Objective To investigate the effect of hydrogen(H2) inhalation on lung injury in rats with severe acute pancreatitis (SAP). Methods Forty-eight healthy male SPF Wistar rats were selected, four groups (12 in each group) were divided into 4 groups by random number table method, sham operation group (SH group), severe pancreatitis group (SAP group), 1%H2 group, 2%H2 group. With the exception of SH group, the rat SAP model was made by injecting 5% sodium taurocholate into each group through retrograde cholangiopancreatine. H2 group 1% and H2 group 2% continuously inhaled H2 at the corresponding concentration during the process of model making and 24 h after successful model making. Blood gas analysis and oxygenation index (PaO2/FiO2) were calculated after 24 h of femoral arterial blood collection after modeling. The rats were then sacrificed to determine the lung wet-to-dry weight ratio (W/D) and the lung tissue injury score. The enzyme linked immunosorbent method was used for determination of levels of 8-hydroxyl deoxyguanosine (8-OHdG), toll-like receptor 4 (TLR-4), nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), IL-6 in the lung tissues. Results Compared with SH group, the W/D ratio of SAP group, 1%H2 group, 1%H2 group were analyzed, the lung tissue damage rating, 8-OHdG, TLR-4, NF-κB, TNF-α, IL-1β, IL-6 and IL-1 increased significantly, PaO2/FiO2 decreased (P ＜ 0.05). Compared with SAP group, W/D ratio, lung tissue damage rating, 8-OHdG, TLR-4, NF-κB, TNF-α, IL-1β, IL-6 and IL-1 in 1%H2 group and 2%H2 group significantly reduced, PaO2/FiO2 elevated (P ＜ 0.05). There was no significant difference in the above indicators between the 1%H2 group and the 2%H2 group (P ＞ 0.05). Conclusion Inhalation of H2 can reduce lung injury caused by severe acute pancreatitis, and the mechanism may be related to inhibition of inflammatory response and oxidative stress.
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