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| The value of circulating blood ANRIL in the early diagnosis of acute myocardial infarction |
| HUANG Ying ZHANG Yun NONG Wenzheng LAN Biyang PAN Rubao ZHANG Dingmin |
| Department of Cardiovascular, Affiliated Ethnic Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning 530001, China |
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Abstract Objective To investigate the value of circulating ANRIL in the early diagnosis of acute myocardial infarction (AMI). Methods Blood samples were collected from 50 AMI patients (experimental group) and 30 patients with coronary angiography normally (control group) from November 2016 to June 2018 in Affiliated Ethnic Hospital of Guangxi Medical University (hereinafter referred to as “our hospital”). Quantitative real-time fluorescence polymerase chain reaction (qRT-PCR) was used to detect the levels of circulating ANRIL at different time intervals (0-6 h, 12 h, 24 h, 7 d and 14 d) after the onset of symptoms of AMI. And the level of plasma cTnT was detected by using electrochemiluminescence． Results There was no significant difference in age, gender, smoking, diabetes, LDL-C, SBP, DBP and Cr between two groups (P ＞ 0.05). There was significant difference in cTnT between two groups (P < 0.05). Compared with control group, the expression level of ANRIL and cTnT in the plasma of AMI patients increased significantly (P < 0.05). In experimental group, the expression level of ANRIL began to increase at the time of admission, decreased after reaching the peak at 12 h, and decreased to the normal range at 7 d. CTnT had a very long diagnostic window period, it started to rise significantly at 6-12 h, then decreased at 24 h, and falled to the normal range at 14 d. ROC analysis showed that when the expression value of ANRIL in 6 h was 3.65 times (the highest value) of the normal control, the sensitivity was 91.9%, the specificity was 91.1%, which was the best diagnostic node; when the cTnT value increased to the highest value of 24 h, the sensitivity and specificity of AMI diagnosis was 91.8%, 92.4% respectively. Conclusion The expression of circulating ANRIL in AMI patients increases significantly and changes dynamically with the course of disease. Compared with cTnT, circulating ANRIL has earlier time window and peak time. It has good early sensitivity and specificity for the diagnosis of AMI.
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