NLRP3炎症小体和氧化应激在新生儿缺氧缺血性脑病患者胎盘组织中的表达及其临床意义

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Abstract Objective To investigate the expression and oxidative stress of NLRP3 inflammasomes in the placenta of neonates with hypoxic-ischemic encephalopathy (HIE), and to investigate the clinical significance of NLRP3 inflammasome and oxidative stress in HIE. Methods From July 2010 to May 2019, 30 cases of HIE placental tissue (HIE group) and 30 cases of normal placental tissue (normal group) were selected from Zengcheng District People′s Hospital, Guangzhou, NLRP3 expression was detected by immunohistochemistry. The concentrations of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured in 10 fresh placental tissues, placental tissues of the two groups were pathologically evaluated. Results The number of villi with cytosygotrophoblast nodules and cytotrophoblast cells in the placenta tissues of neonates in HIE group was higher than that of normal group (P ＜ 0.05). The number of villi with cytosomal membrane was less than that of normal group (P ＜ 0.05). The expression level of NLRP3 in HIE group neonatal placenta was significantly higher than that in normal group, and the difference was statistically significant (P ＜ 0.05). SOD activity in HIE group was lower than that in normal group (P ＜ 0.05), and MDA content was higher than normal group (P ＜ 0.05). NLRP3 was negatively correlated with SOD activity (r = -0.63, P = 0.00), while positively correlated with MDA content (r = 0.74, P = 0.00). Conclusion There is a significant increase in the expression of NLRP3 inflammasomes in the placenta of neonates with HIE and abnormal oxidative stress, both of which are correlated, which may be the cause of neonatal HIE.

Key words： Hypoxic-ischemic encephalopathy NOD-like receptor protein 3 inflammasomes Oxidative stress Placenta

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	WEI Tingyan1
	2 YANG Jie3 ZENG Yuting4 HUANG Qiting4 LIU Yujun4

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WEI Tingyan1,2 YANG Jie3 ZENG Yuting4 HUANG Qiting4 LIU Yujun4. Expression and clinical significances of NLRP3 inflammasomes and oxidative stress in placenta of patient with neonatal hypoxic-ischemic encephalopathy[J]. 中国医药导报, 2020, 17(8): 17-20.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I8/17

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