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| Effect of ellagic acid on cell viability and expression of MEP and SUB genes in trichophyton rubrum |
| LI Zhijian Amina·Abula MA Li MA Fuchang LUO Fuxiang Silafu·Aibai WANG Chao DOU Qin |
| Xinjiang Institute of Traditional Uighur Medicine, Xinjiang Uygur Autonomous Region, Urumqi 830049, China |
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Abstract Objective To investigate the effect of ellagic acid on the cell activity and expression of metalloproteinase (MEP) and subtilis proteinase (SUB) genes in trichophyton rubrum. Methods The experiment was divided into 5 groups, including the growth control group, the terbinanol 0.08 μg/mL group, and the ellagic acid 64, 128 g/mL and 256 g/mL dose groups. After 7 days of drug action, CCK-8 was used to determine the cell activity of trichophyton rubrum, and reverse transcription polymerase chain reaction was used to detect the gene expression levels of trichophyton rubrum protease MEP and SUB gene. Results Compared with the growth control group, the tepinecol 0.08 g/mL and the ellagic acid 64, 128 μg/mL and 256 μg/mL dose groups significantly inhibited the cell activity of trichophyton rubrum, and decreased the expression levels of protease MEP and SUB genes in the fungi, with statistically significant differences (P ＜ 0.05 or P ＜ 0.01). Conclusion Ellagic acid has a strong inhibitory effect on trichophyton rubrum, and can exert its antifungal effect by inhibiting the expression of protease MEP and SUB genes in fungi.
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