组蛋白去乙酰化酶抑制剂CUDC-101对去势抵抗性前列腺癌AR-V7表达的影响

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Abstract Objective To investigate the effect of histone deacetylase inhibitor CUDC-101 on the expression of androgen receptor shear variant 7 (AR-V7) in castration-resistant prostate cancer (CRPC), and to provide relevant theoretical reference for clinical practice. Methods From January 2018 to June 2019, the expression of AR-V7 protein in CRPC PC-3, VCaP, 22Rvl, LNCap and other 17 cell lines were detected by immunoblotting, while the highest AR-V7 expression was screened further the study. According to different inhibitors, the cell lines were divided into MG149 group and CUDC-101 group. The two inhibitors were compared to reduce the expression of AR-V7 in CRPC cells. Then according to different preparation methods, the cell lines were divided into using MDV3100 alone group, 30 nmol dose CUDC-101 combined application group, and 300 nmol dose CUDC-101 combined application group. The effects on the expression of AR-V7 in CRPC cells were analyzed. Results CRPC cell line 22Rvl had AR-V7 positive expression, and PC-3, VCaP and LNCap CRPC cell lines did not find AR-V7 positive expression. The positive rate of AR-V7 expression in the CUDC-101 group was lower than that in the MG149 group, and the difference was statistically significant (P ＜ 0.05). The AR-V7 positive expression rate 30, 300 nmol dose CUDC-101 combined application group were lower than that in the MDV3100 group alone, and the differences were statistically significant (P ＜ 0.05). Conclusion Histone deacetylase inhibitor CUDC-101 can reduce the expression level of AR-V7, while improve the sensitivity of endocrine therapy. It has the potential to treat CRPC.

Key words： Histone deacetylase inhibitors CUDC-101 Prostate cancer Androgen receptor splice variants 7

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	ZHANG Ye ZHANG Lu LIU Xiuheng

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ZHANG Ye ZHANG Lu LIU Xiuheng. Effect of histone deacetylase inhibitor CUDC-101 on expression of AR-V7 in castration-resistant prostate cancer[J]. 中国医药导报, 2020, 17(7): 11-14.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I7/11

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