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| Relationship between high mobility group protein B1 level and helper T cell 17/regulatory T cell balance in patients with primary Sj?觟gren′s syndrome |
| LI Xue1 CHENG Ting1 REN Xiaoying1 WANG Juan1 HOU Ziqi1 LIANG Shufen2 |
1.The Second Clinical Medical College, Shanxi Medical University, Shanxi Province, Taiyuan 030001, China;
2.Department of Clinical Laboratory, the Second Hospital of Shanxi Medical University, Shanxi Province, Taiyuan 030000, China |
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Abstract Objective To investigate the relationship between the level of high mobility group box 1 (HMGB1) and the balance of helper T cell 17 (Th17) /regulatory T cells (Treg) in peripheral blood of patients with primary Sjögren′s syndrome (pSS). Methods Forty patients with pSS in Rheumatology Department of the Second Hospital of Shanxi Medical University from May 2018 to May 2019 were selected as pSS group, and 20 healthy subjects were control group. Elbow venous blood was collected. The number of Th17 and Treg cells was detected by flow cytometry, and Th17/Treg ratio was calculated. Cytometric bead array was used to detect serum interleukin (IL)-17, IL-6 and IL-10 levels. Enzyme-linked immunosorbent was used to detect the levels of HMGB1 and transforming growth factor-β (TGF-β). Each of the above indicators was compared between two groups, and the correlation between HMGB1 and the expression of various cells and cytokines was analyzed. Results The levels of HMGB1, Th17 and Th17/Treg in pSS group were higher than those in control group, while Treg was lower than that in control group, and the difference was statistically significant (P < 0.05). The levels of IL-17, IL-6 and TGF-β in pSS group were higher than those in control group, while IL-10 was lower than that in control group, the differences were statistically significant (P < 0.05). Correlation analysis showed that HMGB1 was positively correlated with Th17, Th17/Treg, IL-17 and IL-6 levels (r = 0.738, 0.502, 0.762, 0.578， all P < 0.05). There was a negative correlation between the levels of HMGB1 and Treg, IL-10 (r = -0.657, -0.686, all P < 0.05), no significant correlation with TGF-β (P ＞ 0.05). Conclusion HMGB1 in peripheral blood of patients with pSS is abnormally high, and there is obvious T cell immune abnormality compared with healthy people, suggesting that HMGB1 may participate in the development of pSS by regulating Th17/Treg balance.
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