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Study on fingerprint of Herba Rhodiolae in Hongba township of Sichuan by HPLC |
SUN Yunbo1,2 SHEN Shuo1,2 ZHANG Chuangfeng1,2 BI Dan1,2 CUI Xusheng3 |
1.Institute of Beijing Yiling Pharmaceutical Co., Ltd, Beijing 102600, China;
2.Hebei Province Institute of Integrated Traditional and Western Medicine, Hebei Province, Shijiazhuang 050035, China;
3.Department of Traditional Chinese Medicine Sources, Shijiazhuang Yiling Pharmaceutical Co., Ltd, Hebei Province, Shijiazhuang 050035 China |
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Abstract Objective To establish high performance liquid chromatography (HPLC) fingerprint of Herba Rhodiolae, and to provide the basis for the establishment of quality standards, and at the same time, to study the quality of different batches of Herba Rhodiolae. Methods RP-HPLC method was used. Waters Xbridge?誖 Shield RP18 (5 μm, 4.6 mm×250 mm) wan used as the column, acetonitrile-water containing 0.1% acetic acid was used as the mobile phase of the gradient elute, with the detection wavelength of 276 nm and the colomn temperature maintained at 30℃. The flow rate was 1.0 mL/min. The recording time was 70 min. Results The HPLC fingerprint method validation met the technical standards. A total of 17 common peaks of Herba Rhodiolae. were found. A total of 12 characteristic peak were identified. They were salidroside, callic acid, caffeic aci, tyrosol, p-coumaric acid, protocatechuic acid, catechin, vanillin, isoquercitrin, quercitrin, chrysophanol 8-O-β-D-glucoside, kaempferol, respectively. The similarity of 13 differernt batches of Herba Rhodiolae. was among 0.863-0.984. Conclusion The specific HPLC fingerprint can be used as a method of scientific evaluation for Herba Rhodiolae. crenulata.
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