miRNA-675-5p与食管鳞癌上皮间质转化的相关性研究

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Abstract Objective To investigated the correlation between the expression of miRNA-675-5p and epithelial-mesenchymal transition (EMT) in esophageal squamous cell carcinoma (ESCC). Methods Real-time quantitative PCR was used to detect the differential expression of miRNA-675-5p in ESCC and normal adjacent tissues, while the correlation between its expression level and clinical stage of patients was analyzed. Liposomes was transfected into ECA109 cell, and divided into miR-675-recursor group, miR-675-5p-inhibition group and NC group. The expression of miRNA-675-5p was up-regulated and inhibited, while the effect of changing the expression of the target gene on the migration and invasion of ECA109 cell lines was analyzed. The expression levels of E-cadherin, N-cadherin, and Vimentin in EMT-associated proteins in each group of cells were detected by western blot experiments, while the effect on the EMT process of ECA109 strain was evaluated. Results Compared with normal adjacent tissues, miRNA-675-5p expression was significantly increased in ESCC tissues (P ＜ 0.05), and comparison of miRNA-675-5p expression in patients with different stages, the differences were statistically significant (P ＜ 0.05). Compared with the NC group, the miR-675-recursor group had significantly enhanced cell migration and invasion ability, and the miR-675-5p-inhibition group had significantly reduced cell migration and invasion ability (P ＜ 0.05). In addition, compared with the NC group, the expression of E-cadherin protein in the miR-675-recursor group was reduced, while the expression of N-cadherin and Vimentin proteins were increased; the expression of E-cadherin protein in the miR-675-5p-inhibition group was increased, while the expression of N-cadherin and Vimentin proteins were reduced (P ＜ 0.05). Conclusion The expression of miRNA-675-5p is related to the TNM stage of ESCC patients. miR-675-5p can promote the interstitial transformation of ESCC cells and thus enhance the invasion ability; down-regulated expression can reverse the EMT process of ESCC cells and reduce the migration and invasion ability.

Key words： Esophageal squamous cell carcinoma ECA109 cells miRNA-675-5p Epithelial mesenchymal transition

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	ZHANG Qian1 SUN Yonghong1 WEN Shimin1 QUAN Yaping1 BIE Jun1 FU Xi2

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ZHANG Qian1 SUN Yonghong1 WEN Shimin1 QUAN Yaping1 BIE Jun1 FU Xi2. Realationship study on miRNA-675-5p and epithelial-mesenchymal transition in esop-hageal squamous cell carcinoma[J]. 中国医药导报, 2020, 17(4): 18-22.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2020/V17/I4/18

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