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| Diagnostic value of combined detection of serum NF-κB, TNF-α levels in patients with tPSA gray area prostate cancer |
| ZHOU Yi WANG Wei LIU Jie YAO Yuan YANG Jianwen |
| Deparment of Urology Surgery, Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology, Guangxi Zhuang Autonomous Region, Liuzhou 545060, China |
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Abstract Objective To investigate the diagnostic value of tumor necrosis factor-α (TNF-α) and nuclear factor-κB (NF-κB) in serum of prostate cancer (PCa) patients. Methods From January 2015 to January 2018, 100 cases of PCa diagnosed and treated in Liuzhou People′s Hospital Affiliated to Guangxi University of Science and Technology (hereinafter referred to as “our hospital”) were selected as the PCa group. Forty patients with benign prostatic hyperplasia (BPH) diagnosed and treated in our hospital during the same period were selected as the BPH group, 40 healthy subjects who underwent physical examination in our hospital during the same period were selected as the healthy control group. Determination of total PSA (tPSA) in each group by electrochemical luminescence. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum TNF-α and NF-κB in each group, and the differences in the expression of each indicator were statistically analyzed. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of each indicator in PCa. Results Comparison of serum tPSA between the BPH group and the PCa group showed statistically significant difference (P ＜ 0.05). The levels of TNF-α and NF-κB in the PCa group were higher than those in the healthy control group and the BPH group, with statistically significant differences (all P ＜ 0.05). The expression of TNF-α and NF-κB in the PCa group was correlated with pathological grade, Gleason score and tumor stage (all P ＜ 0.05), and was independent of age, initial tPSA and the presence of distant metastasis (P ＞ 0.05). Sensitivity and specificity of TNF-α, NF-κB and combined assays were 75.1%, 79.2% and 81.3%, respectively, and 71.5%, 80.1% and 82.4%, respectively. The area under the ROC curve was 0.713, 0.753 and 0.832, respectively. The diagnostic efficiency of joint diagnosis was higher than that of any single indicator. Conclusion The expressions of TNF-α and NF-κB in serum of PCa patients were increased, and the combined detection of TNF-α and NF-κB in serum of PCa patients was of high diagnostic value for PCa in the gray area of diagnosis.
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