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| Expressions, clinical significance and correlation of DNMT1 and NHERF1 in colorectal cancer |
| GAO Xiaobin1 WU Xueliang1 ZHAO Yifeng1 NIE Shuangfa1 LIANG Feng1 LIU Xiaofei2 ZHANG Yingchun1 |
1.Department of General Surgery, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China;
2.Department of Pediatric Surgery, the First Affiliated Hospital of Hebei North University, Hebei Province, Zhangjiakou 075000, China |
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Abstract Objective To explore the expressions, clinical significances and their correlation of DNA methyltransferase-1 (DNMT1) and natrium hydrogen exchanger regulator factor 1 (NHERF1) in colorectal adenocarcinoma tissues. Methods From February 2017 to February 2018, 50 specimens of colorectal adenocarcinoma tissues (cancer group) and 50 specimens of normal colorectal tissues (normal group) in the First Affiliated Hospital of Hebei North University were selected. The expression levels of DNMT1 mRNA and NHERF1 mRNA were detected by real-time fluorescent quantitative reverse transcription polymerase chain (qRT-PCR), and the expression levels of DNMT1 and NHERF1 protein were detected by immunohistochemistry. The clinical data was collected and the relationship between their expression and clinicopathological characteristics was analyzed. The correlation was analyzed by Spearman test. Results The qRT-PCR results showed that the expression level of DNMT1 mRNA in the cancer group was higher than that in the normal group, while the expression level of NHERF1 mRNA in the cancer group was lower than that in the normal group, and the differences were highly statistically significant (all P < 0.01). The immunohistochemical results showed that the positive rate of DNMT1 protein in cancer group was higher than that in the normal group, while the expression level of NHERF1 protein in cancer group was lower than that in the normal group, and the differences were highly statistically significant (all P < 0.01). The positive expression of DNMT1 and NHERF1 protein were correlated with the depth of tumor invasion, TNM stage, lymph node metastasis, and liver metastasis (all P < 0.05), and were not related with gender, age, tumor size and degree of differentiation (all P > 0.05). Spearman correlation analysis showed that there was a negative correlation between DNMT1 and NHERF1 (r = -0.491, P < 0.01). Conclusion The abnormal expression levels of DNMT1 and NHERF1 play an important role in the occurrence and development of colorectal cancer, which can be used as potential biological indicators for early diagnosis and treatment monitoring of colorectal cancer.
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