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| Effect of expression of autophagy related gene Atg7 on replication of hepatitis B virus |
| WANG Yang CHE Yang GAO Yuxue YANG Tongwang ZHANG Yulin CHEN Dexi SHI Ying |
| Beijing Institute of Hepatology, Beijing You′an Hospital, Capital Medical University, Beijing 100069, China |
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Abstract Objective To investigate the effect of Atg7 expression on HBV replication in hepatocytes infected with hepatitis B virus. Methods The expression of Atg7, LC3 and HBs in paraffin section of liver tissue of patients with chronic hepatitis B virus infection in Beijing You′an Hospital from April to May 2019 was observed by immunofluorescence staining. Atg7 overexpression group and Atg7 reduction group were established in HepG2.2.15 cells by using Atg7 expression plasmids and small interfering RNAs. The changes of autophagy and HBV replication level were observed by Western blot and real-time quantitative PCR. Data was analyzed by SPSS 17.0. Results The expression of Atg7 and LC3 in HBs positive cell was higher than that in HBs negative cell; after Atg7 overexpression and starvation, autophagy level, HBc and HBV-DNA expression were higher than those of control group, the replication level of autophagy and HBV increased most significantly in 6 h of starvation, and the differences were highly statistically significant (P < 0.01). The level of autophagy, the expression of HBc and HBV DNA in Atg7 treated cells were lower than those in control group, the decrease was most significant at 6 h after starvation, and the differences were highly statistically significant (P < 0.01). Conclusion HBV infection increases Atg7 expression and autophagy, early intervention of Atg7 expression in autophagy can significantly affect HBV replication.
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