红景天对间歇低氧致大鼠肺损伤的干预作用

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Abstract Objective To investigate the effects of Rhodiolae Herba on pulmonary injury induced by intermittent hypoxia (IH) in rats. Methods Rat model of IH was established. 45 male and Sprague-Dawley rats aged 4 weeks were randomly divided into 5 group according to weight (9 rats in each group): normoxia control group (group A), IH group (group B), IH with low-dose Rhodiolae Herba group (group C), IH with medium-dose Rhodiolae Herba group (group D), IH with high-dose Rhodiolae Herba group (group E). After 45 days, the contents of nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), erythropoietin (EPO), tumor necrosis factor-α (TNF-α) and the expression levels of hypoxia inducible factors-1α (HIF-1α) mRNA and substance P-1 (SP-1) mRNA in the lung homogenate of rats were detected. The pathological changes under microscope were observed, and the relationship between the indicators, oxidative stress and hypoxic reactions were analyzed. Results Compared with group A, the contents of NO and SOD in group B decreased and the content of MDA increased (P < 0.01); compared with group B, the content of SOD in group C increased (P < 0.01), the contents of NO and SOD in group D and group E increased, and the content of MDA decreased (P < 0.05 or P < 0.01). Compared with group A, EPO content and HIF-1α mRNA expression level in group B increased (all P < 0.01); compared with group B, EPO content and HIF-1α mRNA expression level in group C, group D and group E decreased (all P < 0.01). Compared with group A, TNF-α content and SP-1 mRNA expression level in group B increased (P < 0.01); compared with group B, the TNF-α content in group C decreased (P < 0.01), TNF-α content and SP-1 mRNA expression level in group D and group E decreased (P < 0.01). Correlation analysis showed that TNF-α content and SP-1 mRNA level were negatively correlated with NO content (r = -0.55, -0.57), SOD content (r = -0.84, -0.69). TNF-α content, SP-1 mRNA level were positively correlated with MDA content (r = 0.55, 0.56), EPO content (r = 0.69, 0.57), HIF-1α mRNA level (r = 0.86, 0.75) (all P < 0.01). HE results showed that the lung tissue of IH group showed inflammatory and anoxic changes, and the prognosis was improved after intervention. Conclusion Rhodiolae Herba has protective effect on lung injury induced by IH, which may be achieved by antioxidant stress, reducing hypoxia damage and inhibiting inflammatory response.

Key words： Intermittent hypoxia Rhodiolae Herba Oxidative stress Inflammatory response Lung injury

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	WU Maolan1 CAO Hui1 JIAO Ting1 LIU Weiying2

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WU Maolan1 CAO Hui1 JIAO Ting1 LIU Weiying2. Effect of Rhodiolae Herba on pulmonary injury induced by intermittent hypoxia in rats[J]. 中国医药导报, 2019, 16(36): 8-13.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2019/V16/I36/8

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