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| Influence of Bushen Huoxue Decoction on HOXA10 expression in uterine flushing fluid of patients with endometriosis-related infertility |
| LIU Kaili1 TANG Weiwei2 WAN Guiping2 |
1.Graduate School，Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210023, China;
2.Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine, Jiangsu Province, Nanjing 210028, China |
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Abstract Objective To investigate the changes of HOXA10 in uterine flushing fluid after treatment with Bushen Huoxue Decoction in patients with endometriosis (EMs) caused by kidney-deficiency and blood stasis type EMs-related infertility. Methods From December 2016 to June 2018, 20 cases who diagnosed EMs-related infertility as kidney deficiency and blood stasis in the Department of Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine Nanjing University of Chinese Medicine were selected. They were treated with Bushen Huoxue Decoction for three menstrual cycles. The endometrial thickness of the implantation window and blood flow parameters were evaluated by transvaginal Doppler ultrasound before and after treatment. The concentrations of HOXA10 in uterine flushing fluid of before and after treatment were analyzed by enzyme liked imrnunosorbent assay. Results After Bushen Huoxue Decoction treatment, the thickness of the implantation window endometria was obviously higher than that before treatment, blood flow parameters of Uterine artery resistance index (PI) and pulsatility index (RI) were decreased significantly, the expression level of HOXA10 was significantly higher than that before treatment, and the differences were statistically significant (P < 0.05). Condusion Bushen Huoxue Decoction can increase the thickness of endometrium, improve the intrauterine hemodynamic parameters of patients with EMs-related infertility caused by kidney deficiency and blood stasis, improve the expression level of HOXA10 in uterine flushing fluid and improve endometrial receptivity.
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[1] Hudelist G，English J，Thomas AE，et al. Diagnostic accuracy of transvaginal ultrasound for non-invasive diagnosis of bowel endometriosis：systematic review and meta-analysis [J]. Ultrasound Obstet Gynecol，2011，37（3）：257-263.
[2] Eisenberg VH，Weil C，Chodick G，et al. Epidemiology of endometriosis：a large population-based database study from a healthcare provider with 2 million members [J]. BJOG，2018，125（1）：55-62.
[3] Keyhan S，Hughes C，Price T，et al. An Update on surgical versus expectant management of ovarian endometriomas in infertile women [J]. Biomed Res Int，2015，2015：204792.
[4] 刘冬，黄薇.轻度子宫内膜异位症的治疗策略和评价[J].实用妇产科杂志，2012，28（8）：615-617.
[5] Gupta S，Goldberg JM，Aziz N，et al. Pathogenic mechanisms in endometriosis-associated infertility [J]. Fertil Steril，2008，90（2）：247-257.
[6] 韩倩，刘永，赵瑞华.中药影响子宫内膜异位症内膜容受性的研究进展[J].辽宁中医药大学学报，2019，21（7）：205-208.
[7] 李建，周怀君，孔祥怡，等.HOXA10基因在子宫内膜癌组织中的表达及其生物学作用[J].中国肿瘤生物治疗杂志，2018，25（11）：1171-1175.
[8] 杨桂云，刘红林.补肾活血方对子宫内膜异位症不孕腹腔微环境改善作用的实验研究[J].中医药学刊，2003，21（1）：57-59.
[9] 杨桂云.补肾活血汤治疗卵巢功能失调性不孕的临床观察[J].中西医结合学报，2004，2（2）：138-139.
[10] 张莲莲，韩在刚，杨锐.启宫丸对多囊卵巢综合征伴不孕症患者子宫动脉血流和内膜容受性的影响[J].中国药房，2017，28（35）：4967-4970.
[11] 汤伟伟，马小平，朱利，等.补肾活血汤对子宫内膜异位症相关性不孕患者子宫内膜容受性的影响[J].中国医药导报，2016，13（25）：122-124，129，181.
[12] 张建芝，赵少敏，杨欢，等.坤泰胶囊对PCOS不孕症患者应用克罗米芬促排卵子宫内膜的影响[J].河北医药，2014，36（24）：3775-3776.
[13] 胡毅娜，赵琰，丁涛，等.补肾健膜汤改善子宫内膜发育不良患者容受性的临床研究[J].江西医药，2015，50（6）：508-511.
[14] 陈爱兰，白洁，陈晶晶.子宫内膜异位症相关不孕症患者子宫内膜容受性及中药治疗的疗效评价[J].中国中医药信息杂志，2014，21（6）：11-13.
[15] 杨桂云，王佩娟，贾晓斌.“补肾活血汤”对小鼠早期胚胎发育的作用[J].江苏中医，2000（11）：50-51.
[16] 董光苹，严骅.EMs患者子宫内膜容受性特点及中医药对其改善作用[J].辽宁中医药大学学报，2018，20（1）：109-113.
[17] 黄蓉，王玲，宇傲，等.子宫内膜异位症患者子宫内膜PDGF、LN表达及其与血清CA125水平相关性研究[J].实用妇产科杂志，2016，32（2）：113-116.
[18] 吴天思，崔阳阳，孙伟伟，等.活血消异方对子宫内膜异位症模型小鼠子宫内膜容受性的影响研究[J].河北医药，2017，39（7）：965-967，972.
[19] 邓凯贤，柳晓春，郑玉华，等.子宫内膜异位症内膜组织HOXA10基因异常DNA甲基化及意义[J].现代妇产科进展，2011，20（11）：868-872.
[20] 李莉，黄洁明，史云，等.罗氏内异方对子宫内膜异位症模型大鼠内膜容受性的影响[J].新中医，2014，46（8）：182-184.
[21] 汤伟伟，朱利，黄美华，等.补肾活血汤对子宫内膜异位症相关性不孕患者宫腔冲洗液中IL-6与LIF水平的影响[J].吉林中医药，2018，38（11）：1285-1287.
[22] 陈泛泛，谢一红，陈浩波，等.补肾活血汤联合阿司匹林对复发性流产患者子宫动脉血流的影响[J].中国现代医生，2018，56（30）：135-138.
[23] 刘程，朱颍，刘颖，等.HOXA10基因与子宫内膜容受性的研究进展[J].中华临床医师杂志：电子版，2011，5（23）：7073-7075.
[24] 温梦乔，徐琳.HOXA10基因与子宫内膜异位症并不孕症研究进展[J].云南医药，2017，38（5）：529-532.
[25] Taylor HS，VandenHeuvel GB，Igarashi P. A conserved Hox axis in the mouse and human female reproductive system：late establishment and persistent adult expression of the Hoxa cluster genes [J]. Biol Reprod，1997，57（6）：1338-1345.
[26] 戴泽琦，孙伟伟，赵瑞华.近10年针灸影响子宫内膜容受性的国内外研究进展[J].中国针灸，2018，38（4）：451-455.
[27] 周伟，陈莉，曹芳，等.HOXA10及Integrinβ3基因在子宫内膜异位症大鼠子宫内膜组织中的表达及意义[J].世界最新医学信息文摘，2016，16（93）：3-4.
[28] 杨海燕，倪吴花，滕依丽，等.IVF-ET反复种植失败患者子宫内膜HOXA10基因的表达[J].医学研究杂志，2012， 41（10）：76-80.
[29] Lu H，Yang X，Zhang Y，et al. Epigenetic disorder may cause down regulation of HOXA10 in the eutopic endometrium of fertile women with endometriosis [J]． Reprod Sci，2013，20（1）：78-84． |
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