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| Effect of water extract of Acorus tatarinowii on the expression of nitric oxide in human neurogliocyte cell line |
| YANG Haigan1 CAO Rui2 WANG Maojie3 CHEN Yuzhong1 |
Department of Gastrointestinal Surgery, the First Affiliated Hospital of Guangzhou University of Traditional Chinese, Guangdong Province, Guangzhou 510000, China;
2.Department of Critical Care Medicine, the First Affiliated Hospital of Guangzhou University of Traditional Chinese, Guangdong Province, Guangzhou 510000, China;
3.Department of Rheumatology, Guangdong Province Hospital of Chinese Medicine, Guangdong Province, Guangzhou 510000, China |
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Abstract Objective To investigate the effect of water extract from Acorus gramineus on the expression of nitric oxide (NO) in human brain glial cells. Methods LPS was used to stimulate human brain glial cells (HEB) to produce a large amount of NO and induce pathological model. They were divided into control group (phosphate buffer), water extract of Acorus gramineus tatarinus (37.5 μg/mL group), water extract of Acorus gramineus tatarinus (75 μg/mL group), water extract of Acorus tatarinus tatarinus (150 μg/mL group), water extract of Acorus tatarinus tatarinus (300 μg/mL group), water extract of Acorus tatarinus tatarinus (600 μg/mL group), water extract of Acorus tatarinus tatarinus (1000 μg/mL group). The cell activity was detected by methyl thiazolyl tetrazolium and NO was detected by Griess. Results Compared with the control group, the water extract of acorus gramineus tatarinowii had a significant inhibitory effect on HEB cell activity, which was concentration-dependent and time-dependent. The water extract of Acorus gramineus had NO significant effect on the expression of NO in normal HEB cells, but could significantly reduce the lps-induced NO (P ＜ 0.01). Conclusion Acorus gramineus can significantly inhibit the concentration of NO in human brain glial cells induced by LPS, thus protecting brain cells.
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