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| Study on the analgesic effect of extracts from Weigela florida by water |
| WANG Yonghui1 ZHANG Wenzhu1 ZHANG Zirui1 AZIYEERJIANG·Dilinuer1 ZHANG Ying1 LUO Xueya2 |
1.Department of Clinical Medicine, Medical College of Dalian University, Liaoning Province, Dalian 116622, China;
2.Department of Pharmacology, Medical College of Dalian University, Liaoning Province, Dalian 116622, China |
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Abstract Objective To explore the analgesic effect of the extracts of Weigela florida by water in mice. Methods Water extracts of Weigela florida were prepared by soak, extraction, and filtration. Animal models of pain caused by hot plate method and writhing method. A total of 150 clean-grade Kunming mice were selected, and used in both male and female, and hot plate method was used in 50 mice, they were randomly divided into small, medium and large dose groups of Weigela florida water extracts (equivalent to crude drug 0.05, 0.1, 0.2 g/kg), Tramadol Hydrochloride group (0.04 g/kg) and control group (equal volume saline), 10 mice in each group, and administered by intragastric or intraperitoneal injection. The pain thresholds of each groups at 15, 30, 45, 60, 75 min and 90 min after administration were measured. The other 100 animal models with wriggle-induced pain were treated by intragastric or intraperitoneal injection (block method was the same as before), the times of writhing response and inhibition rate of wriggle-induced pain were measured at 11-20 min (T1), >20-30 min (T2), >30-40 min (T3), and >40-50 min (T4) after administration. Results Compared with the saline group, the pain thresholds in the medium dose group at 60 and 75 min, and the large dose group at 15, 45 and 60 min increased significantly, the differences were statistically significant (P < 0.05). Compared with Tramadol Hydrochloride group, there were no statistically significant differences in the medium dose group at 45, 75 and 90 min, the large dose group at 45 and 90 min (P > 0.05), while the differences at other time points were statistically significant (P < 0.05 or P < 0.01). Compared with the saline group, the times of writhing response after the intragastric administration in the small and the large dose group at T1-T4 periods, and the medium dose group at T3 and T4 periods; the times of writhing response after the intraperitoneal injection in the small and the medium dose group at T3 and T4 periods, and the large dose group at T2, T3 and T4 periods decreased significantly, the differences were statistically significant (P < 0.05 or P < 0.01). Compared with Tramadol Hydrochloride group, the times of writhing response after the intragastric administration in the small dose group at T1 and T2 periods, the medium dose group at T1, T2 and T3 periods, the large dose group at T2 period; the times of writhing response after the intraperitoneal injection in the small dose group at T1 and T3 periods, the medium dose group at T1, T2 and T3 periods, the large dose group at T1 period were markedly increased, the differences were statistically significant (P < 0.05 or P < 0.01). Compared with the saline group, the pain threshold of the tramadol hydrochloride group was significantly increased after intragastric or intraperitoneal injection (P < 0.05 or P < 0.01), and the times of writhing response was significantly decreased (P < 0.01). Conclusion Weigela florida water extracts have certain analgesic effects on mice, and the effect is more obvious in the large-dose group. The analgesic effect of tramadol hydrochloride is clear and significant.
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