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| Expression of miR-182 in oral squamous cell carcinoma and its effect on cell biological behaviour |
| WANG FeiFei AN Jinghong SONG Mei WANG Jinhui▲ |
| Department of Clinical Laboratory, the First Hospital of Harbin, Heilongjiang Province, Harbin 150000, China |
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Abstract Objective To investigate the expression of miRNA-182 (miR-182) in oral squamous cell carcinoma (OSCC) and explore the effects of miR-182 deficiency on proliferation and migration in OSCC cells. Methods 10 cases of OSCC samples and paired tissues were collected from Stomatological Hospital of Harbin Medical University from July 2015 to October 2016. The expression of miR-182 in OSCC tissues was detected by quantitative reverse-transcription polymerace chain reaction (RT-qPCR). Culturing TCA8113 cells and separating the samples to four groups: Control, Vehicle, miR-182-NC and miR-182-i group. After transfection with miR-182 inhibitor, TCA8113 cell proliferation and migration were examined with MTT assay and Transwell assay. Results The level of miR-182 in OSCC samples was significantly higher than that in normal paired tissue group (P < 0.05). The proliferation capability and migration capability in miR-182-i group were decreased than those in Control group (P < 0.05). Conclusion The high expression of miR-182 may be correlated with the development of OSCC. Decreased the expression of miR-182 may inhibite OSCC cancer cell proliferation and migration. Further study should be conducted to explore the function of miR-182 in OSCC development.
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