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| Clinical significance of detection of serum hepatitis B virus transcription in maternal and infant vertical transmission |
| ZHAO Wei |
| Department of Gynaecology and Obstetrics, 302th Hospital of PLA, Beijing 100039, China |
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Abstract Objective To explore the clinical significance of the test of serum hepatitis B virus transcription in maternal-infant vertical transmission. Methods From October 2014 to October 2016, 40 maternity patients with positive-HBV surface antigen (HBsAg) and their newborns in 302th Hospital of PLA were selected. All the patients divided into HBeAg positive group (21 cases) and HBeAg negative group (19 cases) according to the HBeAg negative/positive. 2 mL maternal peripheral blood and newborn navel blood were collected, the RNA was extracted, then the HBx DNA, fRNA, trRNA were got through reverse transcription and RT-PCR amplification, the products were showed by agarose gel electrophoresis. Results The positive rates of HBx DNA and fRNA of maternity patients in the HBeAg positive group were higher than those in the HBeAg negative groups, with statistically significant difference (P < 0.05). Then the cases were divided into HBx DNA positive group and HBx DNA negative group according to the HBx DNA negative/positive. The positive rates of fRNA and trRNA of maternity patients and trRNA of newborns in the HBx DNA positive group were higher than those in the HBx DNA negative groups, with statistically significant difference (P < 0.05). Conclusion TrRNA appeared in the process of mother-to-child transmission of HBV is earlier than fRNA, HBx DNA, which is helpful to determine neonatal HBV infection status, and has a certain auxiliary diagnostic value.
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