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| The preparation and evaluation of experimental autoimmune neuritis rat model |
| ZHANG Ming1,2 YUAN Xiaojing3 AO Qiang2 WANG Jianyong2 WEI Yujun2 GONG Kai4 SA Rula1 |
1.School of Clinical Medicine, Inner Mongolia University for Nationalities, Inner Mongolia Autonomous Region, Tongliao 028000, China;
2.Staff Room of Histological Engineering, China Medical University, Liaoning Province, Shenyang 110122, China;
3.Department of Rehabilitation, Tai'an Central Hospital, Shandong Province, Tai'an 250012, China;
4.School of Medicine, Tsinghua University, Beijing 100084, China |
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Abstract Objective To explore the establishment of experimental autoimmune neuritis (EAN) rat model and the evaluation of related indexes. Methods Twenty female Lewis rats weighing 140-160 g were randomly divided into EAN group (n = 10) and control group (n = 10), and the number of each group was 1-10. The EAN group was immunized with P0180-199 peptide. The control group was injected with saline. After 42 days of immunization, the behavioral changes were recorded and scored. At the eighteenth days after immunization, the nerve-muscle action potential, sciatic nerve electronic microscope and immunohistochemistry and HE staining of gastrocnemius muscle and other indicators of the rats in the two groups were detected. Results The sciatic nerve conduction velocity in the peak of the compound action potential in the EAN group was lower than that of control group, with statistically significant difference (P ＜ 0.05), and the amplitude was obviously lower than that of control group, with highly statistically significant difference (P ＜ 0.01). The results of electron-microscope showed swell, honeycomb changes and miss out of myelin sheath, with an attenuation or disappearance of axon in the EAN group. The results of HE staining showed thinning of gastrocnemius-myofibers and stained cytoplasm and other degenerative changes in the EAN group. The results of immunohistochemistry showed that inflammatory factors Iba-1 (macrophage) and CD3 (T cells) were increased in those with swell and miss out of myelin sheath. The axonal protein NF 200 was decreased in those with miss out of myelin sheath. The myelin protein S100 was decreased in those with honeycomb changes and miss out of myelin sheath. Conclusion The autoimmune neuritis model induced by the P0180-199 has high incidence and easy copy, and the pathological changes are close to clinical idiopathic neuritis. In this study, the evaluation indexes of EAN during peak hours provide a basis for the further study of the physiological process of this disease.
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