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| Effects of apigenin combined with ischemic postconditioning on renal ischemia/reperfusion injury in rats |
| LIU Yang LIU Xiuheng WANG Lei DU Yang WANG Zhishun CHEN Zhiyuan GUO Jia |
| Department of Urology Surgery, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, China |
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Abstract Objective To investigate the effects of apigenin combined with ischemic postconditioning on renal ischemia/reperfusion injury in rats. Methods Fifty rats were randomly divided into 5 groups: sham-operated group (sham group), ischemia/reperfusion group (I/R group), apigenin group (api group), ischemic postconditioning group (IPO group) and api+IPO group. The rat model with renal ischemia/reperfusion injury was established, then the animals were sacrificed and nephridial tissue was taken to make paraffin-embedded sections. The pathologic changes of nephridial tissue were observed, the expression of Fas, Caspase-3, Bax were detected by immunohistochemistry, the expression of Fas, Caspase-3, PARP-1 and Bcl-2 were detected by real-time PCR, the expression of Caspase-3, Bax and Bcl-2 were detected by Western blot. Results Compared with the sham group, in I/R group, the pathological tissue showed that kidney tubules were damaged seriously; the immunohistochemistry showed that the expression of Fas and Caspase-3 were enhanced, the expression of Bcl-2 was decreased (P ＜ 0.01); Real-time PCR showed that the expression of Fas, Caspase-3 and PARP-1 mRNA were up-regulated significantly, the expression of Bcl-2 was down-regulated significantly (P ＜ 0.01); Western blot showed that the expression of Caspase-3 and Bax were increased significantly, the expression of Bcl-2 was reduced significantly, the differences were highly statistically significant (P ＜ 0.01). Compared with I/R group, in api+I/R group, IPO group and api+IPO group, the pathological tissue showed that kidney tubules were alleviated; the immunohistochemistry showed that the expression of Fas and Caspase-3 were reduced, the expression of Bcl-2 was increased (P ＜ 0.05); Real-time PCR showed that the expression of Fas, Caspase-3 and PARP-1 mRNA were down-regulated significantly, the expression of Bcl-2 was up-regulated significantly (P ＜ 0.05); Western blot showed that the expression of Caspase-3 and Bax were reduced significantly, the expression of Bcl-2 was increased significantly, the differences were statistically significant (P ＜ 0.05). Conclusion Both apigenin and ischemic postconditioning can reduce the renal ischemia/reperfusion injury, the mechanism may be related to up-regulating the expression of Bcl-2 and down-regulating the expression of Bax, Caspase-3, so as to inhibit the cell apoptosis caused by renal ischemia/reperfusion injury.
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