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| The effect of embryo culture media on the birthweight and length of newborns |
| JIANG Yiqun WANG Shanshan XU Zhipeng ZHANG Ningyuan▲ |
| Reproductive Medicine Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Jiangsu Province, Nanjing 210008, China |
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Abstract Objective To investigate the effect of two commercial embryo culture media (Cook，Vitrolife) on the birthweight and length of newborns after fresh embryo transfer in IVF/ICSI. Methods The data of 1441 patients conceived through IVF/ICSI in Reproductive Medicine Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School from August 2014 to December 2015 were analyzed retrospectively. According to the fetal number and embryo culture media, these patients were divided into two groups: singletons group and twins group, both the two groups included Cook and Vitrolife subgroups. The birthweight and length of newborns were compared in singletons and twins groups. Results There were no significant differences in birthweight and length of the newborns between the Cook and Vitrolife subgroups in singletons group (P > 0.05); while there were no significant differences in birthweight and length between the Cook and Vitrolife subgroups in twins group (P > 0.05). Conclusion It can be concluded that embryos cultured in Cook and Vitrolife media after fresh cleavage embryo transfer do not affect the birthweight and length of newborns.
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[1] Quinn P. Culture systems：sequential [J]. Methods Mol Biol，2012，912：211-230.
[2] Rieger D. Culture systems：physiological and environmental factors that can affect the outcome of human ART [J]. Methods Mol Biol，2012，912：333-354.
[3] Rivera RM，Stein P，Weaver JR，et al. Manipulations of mouse embryos prior to implantation result in aberrant expression of imprinted genes on day 9.5 of development [J]. Hum Mol Genet，2008，17（1）：1-14.
[4] Chang AS，Moley KH，Wangler M，et al. Association between Beckwith–Wiedemann syndrome and assisted reproductive technology：a case series of 19 patients [J]. Fertil Steril，2005，83（2）：349-354.
[5] Dumoulin JC，Land JA，Van Montfoort AP，et al. Effect of in vitro culture of human embryos on birthweight of newborns [J]. Hum Reprod，2010，25（3）：605-612.
[6] Nelissen EC，Van Montfoort AP，Coonen E，et al. Further evidence that culture media affect perinatal outcome： findings after transfer of fresh and cryopreserved embryos [J]. Hum Reprod，2012，27（7）：1966-1976.
[7] Nelissen EC，Van Montfoort AP，Smits LJ，et al. IVF culture medium affects human intrauterine growth as early as the second trimester of pregnancy [J]. Hum Reprod，2013，28（8）：2067-2074.
[8] Kleijkers SH，van Montfoort AP，Smits LJ，et al. IVF culture medium affects post-natal weight in humans during the first 2 years of life [J]. Hum Reprod，2014， 29（4）：661-669.
[9] Eaton JL，Lieberman ES，Stearns C，et al. Embryo culture media and neonatal birthweight following IVF [J]. Hum Reprod，2012，27（2）：375-379.
[10] Vergouw CG，Hanna Kostelijk E，Doejaaren E，et al. The influence of the type of embryo culture medium on neonatal birthweight after single embryo transfer in IVF [J]. Hum Reprod，2012，27（9）：2619-2626.
[11] Balaban B，Brison D，Calderón G，et al. The Istanbul consensus workshop on embryo assessment： proceedings of an expert meeting [J]. Human Reprod，2011，26（6）：1270-1283.
[12] Morbeck DE，Krisher RL，Herrick JR，et al. Composition of commercial media used for human embryo culture [J]. Fertil Steril，2014，102（3）：759-766.
[13] Dyrlund TF，Kirkegaard K，Poulsen ET，et al. Unconditioned commercial embryo culture media contain a large variety of non-declared proteins：a comprehensive proteomics analysis [J]. Human Reprod，2014，29（11）：2421-2430.
[14] Carrasco B，Boada M，Rodriguez I，et al. Does culture medium influence offspring birth weight? [J]. Fertil Steril，2013，100（5）：1283-1288.
[15] Chronopoulou E，Harper JC. IVF culture media：past，present and future [J]. Hum Reprod Update，2015，21（1）：39-55.
[16] Lin S，Li M，Lian Y，et al. No effect of embryo culture media on birthweight and length of newborns [J]. Hum Reprod，2013，28（7）：1762-1767.
[17] Gu F，Deng M，Gao J，et al. The effects of embryo culture media on the birthweight of singletons via fresh or frozen-thawed embryo transfer：a large-scale retrospective study [J]. BMC Pregnancy Childbirth，2016，16（1）：270.
[18] Wunder D，Ballabeni P，Roth-Kleiner M，et al. Effect of embryo culture media on birthweight and length in singleton term infants after IVF-ICSI [J]. Swiss Med Wkly，2014，144：w14038.
[19] Rivera RM，Stein P，Weaver JR，et al. Manipulations of mouse embryos prior to implantation result in aberrant expression of imprinted genes on day 9.5 of development [J]. Hum Mol Genet，2008，17（1）：1-14.
[20] Market-Velker BA，Fernandes AD，Mann MR. Side-by-side comparison of five commercial media systems in a mouse model：suboptimal in vitro culture interferes with imprint maintenance [J]. Biol Reprod，2010，83（6）：938-950.
[21] Feuer SK，Liu X，Donjacour A，et al. Use of a mouse in vitro fertilization model to understand the developmental origins of health and disease hypothesis [J]. Endocrinology，2014，155（5）：1956-1969.
[22] Rexhaj E，Paoloni-Giacobino A，Rimoldi SF，et al. Mice generated by in vitro fertilization exhibit vascular dysfunction and shortened life span [J]. J Clin Invest，2013， 123（12）：5052-5060.
[23] Zhu J，Lin S，Li M，et al. Effect of in vitro culture period on birthweight of singleton newborns [J]. Hum Reprod，2014，29（3）：448-454.
[24] Makinen S，Soderstrom-Anttila V，Vainio J，et al. Does long in vitro culture promote large for gestational age babies? [J]. Hum Reprod，2013，28（3）：828-834. |
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