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Study on the quality standard of Tibetan medicine Corydalis impartiens |
CHEN Weisi1 WANG Jing1,2 YANG Zhonghui3 XIANG Qianben3 SUO Guojia3 CAO Peng2 HUANG Yiping1,2 PENGMAO Cairang3 |
1.Department of Chinese Medicine Preparation, Hospital of Integrated Chinese and Western Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Jiangsu Province, Nanjing 210023, China;
2.Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, National Adninistration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Jiangsu Province, Nanjing 210028, China;
3.Hainan Tibetan Autonomous Prefecture Tibetan Hospital, Qinghai Province, Hainanzhou 813000, China |
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Abstract Objective To establish a quality standard for Tibetan medicine Corydalis impartiens. Methods Ten batches of Corydalis impatiens samples were determined by thin-layer chromatography with protoopioids as reference substance; the moisture content, ash content and extract were determined. The content of protopine in Corydalis impatiens was determined by high performance liquid chromatography with X-Bridge C18 column (250.0 mm×4.6 mm, 5 μm) and acetonitrile-triethylamine acetic acid solution (20∶80) as the mobile phase, meanwhile high performance liquid chromatography fingerprint was established and the similarity was calculated. Results The thin-layer chromatographyresults of 10 batches of Corydalis impartiens had one accurate characteristic spot. The original opioid has a good linear relationship in the mass concentration range of 11.71-585.80 μg/mL, the average recovery rate was 99.84% and the RSD was 2.75%. By high performance liquid chromatography fingerprint analysis, 14 common characteristic peaks were identified, and the similarity was above 0.9. Conclusion The method is simple, repeatable, which can be used for the quality control of Corydalis impatiens.
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