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| Investigation on the normal reference range of healthy children serum Vitamin B12 in Guangzhou |
| HUANG Yujun1 WU Shaoguo2 QIU Yuanyuan3 CAI Minmin4 CHEN Huimin5 LI Yanyou6 |
1.Department of Laboratory Medicine, Guangzhou Children's Hospital, Guangdong Province, Guangzhou 510120, China;
2.Department of Laboratory Medicine, Guangzhou 12th People's Hospital, Guangdong Province, Guangzhou 510620, China;
3.Department of Laboratory Medicine, Guangzhou Maternity Health Care Center, Guangdong Province, Guangzhou 510120, China;
4.Department of Laboratory Medicine, Zhujiang New Town Branch of Guangzhou Women and Children Medical Center, Guangdong Province, Guangzhou 510620, China;
5.Department of Clinical Nutrition, Zhujiang New Town Branch of Guangzhou Women and Children Medical Center, Guangdong Province, Guangzhou 510620, China; 6.Tianjin Lanbiao Science and Technology Ltd., Tianjin 300384, China |
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Abstract Objective To establish the normal reference range of healthy children serum Vitamin B12 (VB12) in Guangzhou. Methods Levels of serum VB12 of 2065 healthy children (1208 boys and 857 girls) and 129 adults (52 male and 77 female) living in Guangzhou Region were examined by LK3000V Vitamin Analyzer from September 2015 to December 2016. The results were analyzed statistically. Results There were no statistical differences in levels of serum VB12 between boys and girls in 11 age groups (aged less than 11) and 11-17 age group (P > 0.05). The median (P2.5, P97.5) levels of serum VB12 defined by age were 252.4 (192.3, 338.0) pg/mL of 0-2 years-old group, 253.1 (192.5, 359.5) pg/mL of >2-5 years-old group as well as >7-17 years-old group, 262.7 (194.8, 374.9) pg/mL of >5-7 years-old group, 297.5 (233.7, 383.1) pg/mL of ＞17 years-old group, there were statistically significant differences between any two groups (P < 0.05). Positive correlation was found between age and level of serum VB12 (r=0.228, P=0.000). Conclusion Reference ranges of healthy children serum VB12 in Guangzhou Region are showed as follows: 192.3-338.0 pg/mL of 0-2 years-old children, 192.5-359.5 pg/mL of >2-5 years-old as well as >7-17 years-old children, and 194.80-374.9 pg/mL of >5-7 years-old children.
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