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| Effects of different dose of Propofol on hypothalamus, pituitary, hippocampus Akt and amyloid β protein of rats |
| XIE Hengtao PAN Xia HE Xuan LIU Kang WANG Long▲ |
| Department of Anesthesiology, Renmin Hospital of Wuhan University, Hubei Province, Wuhan 430060, China |
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Abstract Objective To observe the effects of different Propofol anesthesia doses on rat hypothalamus, pituitary, hippocampus Akt and amyloid beta protein (Aβ). Methods 24 SPF grade male rats were used by method of low-dose tail intravenous injection (10 mg/mL) for 3 times. The rats were divided into 4 groups by methods of cumulative dose, control, Propofol group Ⅰ (10 mg/mL), Propofol group Ⅱ (20 mg/mL) and Propofol group Ⅲ (30 mg/mL), respectively. No anaesthetic management was given in control group, with 6 rates in each group. Electroencephalogram was detected by electroencephalograph; the pathology of rat hypothalamic arcuate nucleus neurons and pituitary gonadotropin cell was observed by HE staining. Western blot detection was used to detect the expression of the Akt and Aβ. Results Compared with the control group, the spike wave, α wave and β wave increased significantly with the increase of the Propofol dose (P < 0.05), and the δ wave decreased significantly with the Propofol dose increasing (P < 0.05). The density and distribution of hypothalamic arcuate nucleus were normal in the control group, with the large whole number of adenohypophysis cells, abundant sinusoid capillary between cells, and the cells were arranged regularly; no obvious lesions of rats arcuate nucleus neuron in Propofol group Ⅰ was observed, as well as no obvious reduction in adenohypophysis cells. With increasing of dose of anesthesia, the number of hypothalamic neuron cells and adenohypophysis cells reduced significantly, with unclear boundary of nuclear membrane and an increase in the number of irregular arrangement cells. The Western blot results suggested that with increasing of the Propofol dose, the Akt hippocampus protein expression reduced dramatically (P < 0.01) while Aβ expression increased significantly (P < 0.01) in anesthesia group comparing with control. Conclusion Low-dose Propofol anesthesia can not cause lesions in hypothalamus and hypophysis, but their feedback effects enhanced with Propofol dose increasing, causing pathological changes. The deposition effects of Propofol anesthesia on Aβ may be achieved by inhibiting Akt pathway.
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