枸杞多糖对中波紫外线辐射后角质形成细胞炎症因子表达的影响

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Abstract Objective To study the effects of the Qinghai Tsaidam Basin Lyciumbarbarum polysaccharide(LBP) on the level of interleukin1 (IL-1) and interleukin6 (IL-6) in cultured human immortalized keratinocytes (HaCaT cells) after ultraviolet (UVB) irradiation. Methods HaCaT cells were cultured in vitro and randomly divided into five groups: control group, UVB radiation group (30 mJ/cm2, radiation 40 min), LBP low dose group (0.5 mg/mL, 30 mJ/cm2,radiation 40 min) , LBP middle dose group (1 mg/mL, 30 mJ/cm2, radiation 40 min) , LBP high dose group(2 mg/mL, 30 mJ/cm2, radiation 40 min). Before 12 h of UVB irradiation, LBP was added into the culture medium. Enzyme-linked immunosorbent assay (ELISA) was applied to test the effects of LBP on groups of cells and the supernatant of the level of IL-1 and IL-6. Results Compared with the control group, the levels of IL-1 in the cell in the UVB radiation group had no significant change (P ＞ 0.05), the level of IL-6 in the cell was significantly higher (P < 0.05). The levels of IL-1 and IL-6 in supernatant were significantly increased (P < 0.05). Compared with UVB radiation group, LBP group of cells or in the supernatant IL-1 and IL-6 levels were significantly reduced (P < 0.05). Conclusion Qinghai Tsaidam Basin LBP can reduce synthesis and secretion of inflammatory factors in HaCaT cells after UVB radiation factor, and reduce the skin damage caused after ultraviolet radiation.

Key words： Lyciumbarbarum polysaccharide Human immortalized keratinocytes Ultraviolet radiation Interleukin 1 Interleukin 6

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	ZHANG Chen YANG Ya JIA Yang'e REN Licuan YAN Hualing

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ZHANG Chen YANG Ya JIA Yang'e REN Licuan YAN Hualing. Effect of Lyciumbarbarum polysaccharide on expression of inflammatory cytokines in keratinocytes after UVB irradiation[J]. 中国医药导报, 2017, 14(19): 20-23.

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http://www.yiyaodaobao.com.cn/EN/ OR http://www.yiyaodaobao.com.cn/EN/Y2017/V14/I19/20

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