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| Determination of plasma catecholamine and its metabolites in hereditary pheochromocytoma family |
| LIU Hongxu1 DENG Sishan1 MA Lihong1 MA Zhenjing1 WANG Xinkang2 |
1.Fujian Academy of Medical Sciences, Fujian Provincial Key Laboratory of Medical Testing, Fujian Province, Fuzhou 350001, China;
2.Clinical Medical College of Fujian Medical University, Department of Electrocardiogram of Fujian Provincial Hospital, Fujian Province, Fuzhou 350001, China |
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Abstract Objective To establish a method for the determination of catecholamines and their metabolites in plasma of hereditary pheochromocytoma families by high performance liquid chromatography-electrochemical detector (HPLC-ECD). Methods The working electrode was a glass electrode and the reference electrode was an Ag-AgCl electrode with a voltage of 0.6 V and a temperature of 30℃. Using Ultimate XB-C18 (4.6 mm × 150 mm, 5 μm); using methanol as mobile phase A, 0.1% sodium dihydrogen phosphate (containing EDTA-Na and octanesulfonic acid) aqueous solution as mobile phase B, gradient elution, flow rate 0.8 mL/min; column temperature was room temperature; detection wavelength was 280 nm; injection volume was 20 μL. Results In normal healthy people, the contents of epinephrine(E)， norepinephrine(NE), methoxy norepinephrine (NMN), methoxy-adrenalin (MN) were (56.71±25.67), (98.62±36.90), (78.36±22.36), (33.27±14.62) ng/L. The NEs of 2 patients with pheochromocytoma diagnosed in pheochromocytoma were 353.36, 563.87 ng/L and NMN were 425.71 and 527.65 ng/L, respectively. The correlation detection values of the other 18 family members were within the range of normal healthy adults. The correlation detection values of the other two family members were higher than the normal values. it is recommended to go to the hospital for further examination. Conclusion This method is simple, sensitive and accurate and can be used for the detection of patients with clinical pheochromocytoma.
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